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清肺化痰通络方对大鼠肺纤维化的干预作用及对TGF-β1表达的影响(1)
http://www.100md.com 2012年4月1日 赵敏,陈忻怡,陈振发
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     摘 要:目的:观察清肺化痰通络方对肺间质纤维化大鼠肺内胶原纤维沉积和转化生长因子-β1(TGF-β1)表达的影响。方法:Wistar雌性大鼠72只,随机分为对照组、模型组、中药组。模型组和中药组大鼠气管内一次性滴注博莱霉素A5(BLMA5),对照组大鼠气管内一次性滴注等体积的生理盐水。造模后2h开始给药,模型组和对照组灌服生理盐水,中药组灌服清肺化痰通络方药物,每天一次。各组动物分别于第3、7、14、28天各处死6只,取肺组织进行HE和Masson染色,观察肺内胶原的沉积部位和数量;同时进行免疫组化染色,结合图像分析来检测各组肺组织中TGF-β1的表达水平。结果:对照组肺内结构正常,胶原沉积不明显;模型组可见支气管黏膜下、血管壁及其周围、肺间质、肺泡壁有大量胶原沉积;中药组胶原沉积明显轻于模型组。第14天模型组、中药组TGF-β1在肺内的表达呈强阳性,高于对照组,差异有显著性意义(P<0.01)。中药组TGF-β1表达数量少于同一时段模型组,差异有显著性意义(P<0.05)。结论:清肺化痰通络方可以抑制肺间质纤维化大鼠肺组织内胶原的沉积,可以减缓肺纤维化的形成,其作用机制可能是通过抑制TGF-β1的表达来实现。

    关键词:肺纤维化;胶原;TGF-β1

    中图分类号:R285.5

    文献标识码:A

    文章编号:1673-7717(2012)04-0869-03

    Influence of Qingfei Huatan Tongluo Decoction on Pulmonary

    Fibrosis and TGF-β1 Expressionin in Rats

    ZHAO Min,CHEN Xin-yi,CHEN Zhen-fa

    ( Basic Medical Sciences,Hubei University of Traditional Chinese Medicine,Wuhan 430061,Hubei,China)

    Abstract:Objective: To observe the influence of Qingfei Huantan Tongluo Decoction on pulmonary fibrosis and tgf-β1 expressionin in rats.Methods:72 Wistar female rats were randomly divided into control group,model group and Chinese medicine group. Rats in the model group and the Chinese medicine group were given intratracheal instillation of bleomycin A5(BLMA5) once only,rats in the control group were given endotracheal infusion of isometrical physiological saline. Two hours after building the model,the model group and the control group were given intragastric administration of physiological saline,Chinese medicine group was intragastric administration of Qingfei Huatan Tongluo prescription drugs,once a day. 6 rats of each group were respectively killed on the third,seventh,fourteenth,twenty-eighth day,and HE and Masson staining for lung tissue was used for observation of lung collagen deposition site and number;at the same time immunohistochemical staining combined with image analysis was used to detect TGF-β1 expressions of lung tissue in groups. Results:In the control group ......

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