摘 要：目的 了解荧光定量PCR用于检测成人社区获得性肺炎患者肺炎支原体意义。方法：对50例社区获得性肺炎患者痰和咽拭及27例无呼吸道感染健康人痰进行荧光定量PCR检测肺炎支原体DNA。结果：健康人27例中8例PCR阳性，CAP患者中45例留痰标本，其中27例做PCR，全部阳性，咽拭49例，36例阳性。以健康人痰中DNA含量高值为感染阈值，最后确定感染比例50%。结论：健康人中上呼吸道存在肺炎支原体DNA，而荧光定量PCR可以作为肺炎支原体检测方法且检测时间短。

    关键词：社区获得性肺炎；荧光定量PCR；肺炎支原体；咽拭子；痰

    Abstract：ObjectiveTo know the value of flurescence quantitative polymerase chain reaction (FQ-PCR) that detect the Macoplasma pneumonia of the patiant who are the community acquired pneumonia. Method A total of 50 patients with community-acquired pneumoniae and the heathy control group 27 persons whose respiratory secretion will be check by flurescence quantitative polymerase chain reaction (FQ-PCR).Result:Eight of 27 persons in the group is positive .There are 45 persons who generate sputums,27do the flurescence quantitative polymerase chain reaction ,and all are posive. The swab are 49 ,and 36 are posive.Ti is the standard that make sure which is affected by the MP.The threshold is the top of the the health people respiratory secretion quility of the MP DNA.The rate of infection is 50%.Conclusion:There are MP DNA in the upper respiratory tract of the health people.The flurescence quantitative polymerase chain reaction (FQ-PCR) is a fast and valubale mean to test the DNA of MP. ......