绞股蓝皂甙对Aβ诱导AD细胞模型的影响(1)
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摘要：目的 探讨绞股蓝皂甙对Aβ诱导AD细胞模型的影响。方法 原代培养胎鼠基底前脑胆碱能神经元，实验细胞分为6组：空白对照组、BDNF组、SLs组、β淀粉样蛋白140(Aβ140)组、Aβ140+BDNF组和Aβ140+SLs组。应用MTT比色试验检测SLs对细胞活性的影响，免疫细胞化学染色检测胆碱乙酰转移酶(ChAT)的表达；采用Aβ140诱导AD细胞模型，运用免疫细胞化学染色和流式细胞仪分别检测诱导型一氧化氮合酶(iNOS)的表达和凋亡。结果 空白对照组、BMDF组、SLs组、Aβ140组、Aβ140+BNDF组、Aβ140+SLS组实验细胞MTT检测细胞积分吸光度(IA)值分别为0.61±0.04、0.91±0.07、0.92±0.07、0.36±0.02、0.56±0.04、0.55±0.04，ChAT免疫阳性细胞数分别为43.00±2.73、64.00±4.57、61.00±4.62、21.00±1.92、41.00±2.69、40.00±2.54，iNOS免疫阳性细胞数分别为41.00±2.92、40.00±2.89、40.00±2.96、67.00±5.26、46.00±2.78、45.00±2.97，细胞凋亡数分别为30.00±2.31、29.00±2.12、28.00±2.49、57.00±3.83、33.00±1.75、34.00±3.46。Aβ140+SLs组细胞活性与ChAT阳性细胞数均高于Aβ140组(P<0.05)，Aβ140+SLs组iNOS阳性细胞数与细胞凋亡数均低于Aβ140组(P<0.05)。结论 SLs可促进体外培养的胆碱能神经元及其突起生长，可提高神经元的活性和ChAT表达，并可有效抑制Aβ140诱导的神经元iNOS表达和细胞凋亡。绞股蓝皂甙对Aβ诱导的AD细胞模型具有保护作用。
关键词：阿尔茨海默病；绞股蓝皂甙；胆碱乙酰转移酶；诱导型一氧化氮合酶；细胞凋亡
中图分类号：R742 文献标识码：A 文章编号：16721349(2012)02020104
The Influence of Steven Leafs on the Cell’s Model of AD Inducted by Aβ140
Zheng Yanli,Sun Tianmin,Wang Meng,et al // Renmin Hospital,Hubei University of Medicine (Shiyan 442000)
Abstract:Objective To explore the effects of stevenleafs (SLs) on the cells model of Alzheimer’s disease inducted by amyloid beta peptide 140(Aβ140).Methods Basal forebrain cholinergic neurons from fetal rats were primarily cultured.The experimentation cells were divided into the blank control group,brain derived neurotrophic factor (BDNF) group,SLs group,Aβ140 group,Aβ140+BDNF group,and the Aβ140+SLs group at random.The cells were observed under inverted microscope and the cells viability was assessed using MTT assay.The expression of choline acetyltransferase (ChAT)positive neurons was detected by immunocytochemical staining. Aβ140 was added.The expression of inducible nitric oxide synthase (iNOS) in the cells with or without SLs pretreatment was observed by immunocytochemical staining.The cells apoptosis was detected by flow cytomertry.Results MTT assay showed that incubation of the cells with SLs resulted in the highest cell viability,which was incomparable with that cell incubated in the presence of BDNF (P>0 ......
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