doi：10.12102/j.issn.1672-1349.2025.14.009

    The Effect DNMT1on Myocardial Fibrosis in Ratswith Chronic Heart Failure Models CHENGNa，HANJing，SHANZimei

    ， ，， CorrespondingAuthorSHAN Zimei，E-mail：1641597337 @ qq.com

    AbstractObjective：ToexploretheefectDNAmethyltransferase1(DN1onmyocardialfibrosisinratswithchronicheartfailure (CHF)modelsandismolecularmechanismMethods：The SDratswererandomlydividedintoShamoperation(Sham)group，CHFgroup， si-NCgroupandsi-DNMT1group.ExceptfortheShamgroup，theCHFmodelswerereplicatedthroughabdominalaorticcoarctation.The abdominalaortatheratsintheShamgroupwasisolatedbutnotligatedAftertheoperationthesi-NCgroupandthesi-DN1group wereinjectedwithngativecontroentivirusandDsiNArecombinantentivirusthroughmyocarduminjectionrespectielye leftentricularjefracti)，fttriculareate(，fttriculastolameterdl ventricularend-systolicdiameterLVESD)ratsineachgroupweredeterminedbyDopplerulrasoundTehistopathologicalchanges myocardialtissesratsineachgroupwereobservedbyhematoxylin-eosin(HE)staining.Masonstainingwasusedtooservethe myocardialtissefibrosis.Theexpressionsmoothmuscleactin(-SMA)inmyocardialtissuesratsweredetectedby immunuoresecsainnexpresoeflndliadalisssrtseeeeti fluorescencequantativepolymerasechainreaction(qRT-PCR)andWestemBlotResults：ComparedwiththeShamgroupthereltive expression levels DNMT1mRNA andprotein in themyocardial tissue CHF group wereupregulated Plt;0.05) ......