排铅冲剂驱铅的实验研究
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摘要：目的研究排铅冲剂对染铅小鼠的驱铅作用。方法选用50只18～22 g雄性小鼠，随机分为空白对照组、模型对照组和3个实验组。低、中、高剂量组和模型组自由饮用0.1%的三水醋酸铅水溶液；低、中、高剂量组分别灌胃2，4，8 g/kg BW剂量的受试药液，模型对照组和空白对照组灌胃去离子水，连续30 d。采血，取肝、股骨，用石墨炉原子吸收光谱法测定铅含量。结果3个实验组小鼠肝组织铅含量显著低于模型对照组，但各剂量组小鼠血和骨铅含量无明显影响。结论排铅冲剂有排铅作用。
关键词：排铅冲剂；排铅；原子吸收光谱
中图分类号： R995文献标识码： A文章编号：1672-979X(2007)10-0013-03
Experiment Study on Lead-removing Effect of Paiqian Granules
DENG Li-juan1, CHEN Ya-fei1, JIANG Lu2, FAN Hui-hong1
(1. National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China;
2. China Agricultural University, Beijing 100083, China)
Abstract：Objective To study the lead-removing effect of Paiqian Granules in lead-exposed mice. Methods Fifty male mice weighed from 18g to 22g were randomly divided into five groups of blank control, model control and low, middle, high dose groups. In model control and low, middle, high dose groups, the mice freely drank 0.1% lead acetate trihydrate solution. Then, the mice in low, middle, high dose groups were additionally administered with Paiqian Granules by gavage for 30 days at doses of 2, 4, 8g/kg BW, respectively. The mice in blank control and model control groups were given deionized water for 30 days. At the end of the experiment, samples of whole blood, liver and femur were collected for lead detection by GF-AAS. Results Compared with model control group, the lead content in the liver of mice in low, middle, high dose groups significantly decreased. But, there was no significant difference for the lead content in the blood or femur of mice in all groups. Conclusion Paiqian Granules can promote lead excretion in mice.
Key words：Paiqian Granules; lead-removing; atomic absorption spectrometry(AAS)
排铅冲剂是以绿茶、金钱草、黄芪、木瓜、甘草和乳酸钙为主要原料制成的保健食品，具有促进排铅的作用。为验证本品排铅的功效，对排铅冲剂进行了驱铅实验。
1材料与仪器
1.1受试物
排铅冲剂为黄白黑混合砂状粉末，溶于水，规格6 g /袋，批号20040210，常州市红太阳生物工程有限公司生产。口服推荐量为每人24 g/d，以每人60 kg体重计算，折合0.4 g/kg BW。
1.2动物及饲养
SPF级昆明种小鼠，雄性，体重18~22 g，共50只，由中国药品生物制品检定所实验动物中心提供(动物质量合格证SCXK京2005-0004号)。小鼠在二级实验动物室随机分笼，每笼10只，自由摄食无菌颗粒块料，饮水，饲养观察1周，用于实验。实验动物室温22~24 ℃，相对湿度40%~45%，日光灯照明 12 h。塑料鼠盒体积29 cm×18 cm×2 cm ......
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