摘要：目的研究聚合度6～8的壳寡糖的制备工艺。方法选用合适的酶降解壳聚糖，超滤、干燥；用TLC、HPLC、MALDI-TOF-MS检测产品。结果产品为白色，主要为聚合度6～8的壳寡糖。结论此工艺能用于制备聚合度主要为6～8的壳寡糖。

    关键词：壳寡糖；聚合度；制备；基体辅助激光解吸电离-飞行时间质谱法

    中图分类号：Q53文献标识码：A文章编号：1672-979X(2008)03-0014-03

    Preparation of Chitooligosaccharides with Degree of Polymerization of 6 to 8

    CHEN Mian, ZHU Xi-qiang*, Li Zhi-ming, GUO Xue-ping

    (Institute of Biopharmaceuticals of Shandong Province, Jinan 250108, China)

    Abstract：Objective To study the preparation technology of chitooligosaccharides with degree of polymerization of 6~8. Methods Chitosan was hydrolyzed by special enzymes, then the hydrolysis product was successively ultrafiltered, dried and examined with TLC, HPLC and MALDI-TOF-MS. Results The product was white powder and mainly contains chitooligosaccharides with degree of polymerization of 6~8. Conclusion This method can be used for the preparation of chitooligosaccharides with degree of polymerization of 6~8. ......