HPLC法测定复方黄松洗液中蛇床子素的含量
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[摘要] 目的：建立HPLC法测定复方黄松洗液中蛇床子素的含量。方法：采用SB-C18分析柱(4.6 mm×250 mm，5 μm)；流动相：乙腈-水(65∶35)；检测波长：322 nm。结果：蛇床子素在0.053~1.060 μg范围内线性关系良好(r=0.999 9)，平均回收率为98.5%，RSD为3.6%。结论：本法操作简单、灵敏、准确，可用于复方黄松洗液的质量控制。
[关键词] 复方黄松洗液；蛇床子素；HPLC
[中图分类号] R917[文献标识码]A [文章编号] 1673-7210(2007)12(c)-163-02
Content determination of osthole in Compound Huangsong Lotion by HPLC
TAN Shi-xin, WANG Xiao-bing
(1.Nanjing Municipal Hospital of Traditional Chinese Medicine, Nanjing210001,China;2.The People’s Hospital of Jiangsu Province, Nanjing210029,China)
[Abstract] Objective: To establish the HPLC method for content determination of osthole in Compound Huangsong Lotion. Methods: The SB-C18 column(4.6 mm×250 mm,5 μm) was used,the mobile phase consisted of acetonitrile-water(65∶35) and the detective wavelength was at 322 nm ......
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