[摘要] 目的：建立HPLC法测定五柏参洗液中盐酸小檗碱含量的方法。方法：色谱柱为DiamonsilTM(钻石) C18(200 mm×4.6 mm，5 μm)，以乙腈-0.033 mol/L磷酸二氢钾溶液(40∶60) 为流动相，检测波长为345 nm，流速为1.0 ml/min。结果：盐酸小檗碱在10.87~108.70 μg/ml浓度范围内线性关系良好。平均回收率为99.67%，RSD=0.12%。结论：本法简便、灵敏，结果准确可靠，可用于五柏参洗液中盐酸小檗碱的含量测定。

    [关键词] 五柏参洗液；盐酸小檗碱；高效液相色谱法

    [中图分类号]R917 [文献标识码]A [文章编号]1673-7210(2008)04(c)-019-02

    Content determination of berberine hydrochloride in Wubaishen Cleaning Solution by HPLC

    TONG Shao-hui1, LI Na2, CAI Yu-hua3

    (1.Dandong Institute for Drug Control, Dandong 118002, China; 2.Dandong Traditional Chinese Medicine Hospital, Dandong 118000, China; 3. Dandong Yichuang Parmaceutical Company Limited, Dandong 118002, China)

    [Abstract] Objective: To develop an HPLC method for the content determination of berberine hydrochloride in Wubaishen Cleaning Solution.Methods: The DiamonsilTM C18 column (200 mm×4.6 mm ......