乙肝患者血清中HBeAg与乙肝病毒DNA关系的探讨
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[摘要] 目的：探讨乙肝患者血清中e抗原(HBeAg)与乙肝病毒DNA(HBV-DNA)之间的关系。方法：选取305例HBsAg阳性的乙肝患者血清标本，分别用微粒子酶免疫分析法(MEIA)和核酸扩增荧光定量法(FQ-PCR)检测HBeAg和HBV-DNA。结果：305例标本中，HBeAg阳性率为71.48%，而HBV-DNA阳性率为58.69%。HBeAg阳性标本中，HBV-DNA阳性率为66.05%；而HBeAg阴性标本中，HBV-DNA阳性率为40.23%，两者有显著性差异(?字2=17.11，P<0.01)。不同浓度组HBeAg和HBV-DNA检测结果比较，有统计学意义(?字2=35.67，P<0.01)。结论：HBV-DNA比HBeAg能更准确反映出乙肝病毒复制情况，可以更好地指导临床进行乙肝治疗。
[关键词] 乙型肝炎；HBeAg；HBV-DNA
[中图分类号]R512.6 [文献标识码] B[文章编号] 1673-7210(2009)02(b)-050-02
The relationship between serum HBeAg and HBV-DNA in the patients infected hepatitis B virus
LI Meng-hua，SHI Long
(Department of Clinical Laboratory,the Sixth People's Hospital of Dalian, Dalian116000,China)
[Abstract] Objective:To explore the relationship between serum HBeAg and HBV-DNA contents. Methods:305 serum samples which have shown HBsAg positive were included.HBeAg was tested by microparticle enzyme immunoassays(MEIA)and HBV-DNA was tested by fluorescence quantitative polymerase chain reaction (FQ-PCR). Results: 71.48% of 305 samples showed HBeAg positive, while 58.69% samples showed HBV-DNA positive.In the HBeAg positive samples,the positive percentage of HBV-DNA was 66.05%,and in the HBeAg negative samples,the positive percentage of HBV-DNA was 40.23%,which showed there was significant difference(?字2=17.11，P<0.01)between HBeAg and HBV-DNA.The differences between HBeAg in a series of concentration and the test results of HBV-DNA were also significant(?字2=35.67，P<0.01). Conclusion: Compared with HBeAg,HBV-DNA is a better maker for detecting the replication of HBV(hepatitis B virus),which would be helpful for the HBV clinical therapy.
[Key words] Hepatitis B;HBeAg;HBV-DNA
目前我国有慢性无症状乙肝病毒携带者约1.2亿人，慢性乙肝患者约3 000万人，这已经成为我国最严重的公共卫生安全问题之一，给社会和家庭带来沉重的经济负担。现在对HBV的HBV-DNA检测比HBeAg能够更准确反映出乙肝病毒复制情况及是否具有传染性。笔者通过对HBsAg阳性血清进行HBeAg和HBV-DNA的检测，探讨两者之间关系，现将结果报道如下：
1 资料与方法
1.1标本来源
2008年6月我院门诊和住院乙肝病例305例，HBsAg均阳性，其中，男232例，女73例，年龄6～68岁，平均(39±12.72)岁。
1.2检测方法
MEIA法采用美国Abbott公司生产AXSYS·SYSTEM全自动免疫分析仪，试剂由该公司配套提供，结果判定时HBeAg≥1 S/CO为阳性。FQ-PCR法采用美国AB公司生产7500型实时荧光PCR系统，试剂由上海克隆生物高技术有限公司提供，结果判定时HBV-DNA≥1 000 IU/ml为阳性 ......
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