RP-HPLC法测定酮甲霜中酮康唑和甲硝唑的含量
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[摘要] 目的:建立反相高效液相法测定酮甲霜中酮康唑和甲硝唑含量的方法。方法:采用Diamonsil-C18(4.6 mm×250 mm,5 μm)色谱柱;流动相:甲醇-水(74∶36);检测波长:239 nm;流速:1.0 ml/min。结果:酮康唑在0.06～2.10 μg范围内与峰面积呈良好的线性关系(r=0.999 5),平均回收率为98.89%,RSD=1.40%;甲硝唑在0.06～2.10 μg范围内与峰面积呈良好的线性关系(r=0.999 1),平均回收率为98.75%,RSD=1.10%。结论:本方法简便、准确、重现性好,可用于酮甲霜中酮康唑和甲硝唑的质量控制。
[关键词] 反相高效液相法;酮甲霜;酮康唑;甲硝唑;含量测定
[中图分类号] R927.2[文献标识码] B[文章编号] 1673-7210(2010)04(c)-079-02
Content determination of ketoconazole and metronidazole in Compound Ketoconazole Cream by RP-HPLC
WANG Xiaoyan1, GAO Dan2
(The PLA 230th Hospital, Dandong 118000, China)
[Abstract] Objective: To establish a method for the content determination of ketoconazole and metronidazole in Compound Ketoconazole Cream by RP-HPLC. Methods: The Diamonsil C18 (4.6 mm×250 mm, 5 μm) column was used. The mobile phases consisted of acetonitrile and water (74∶36), the flow rate was 1.0 ml/min and the detection wavelength was at 239 nm. Results: The linear ranges of ketoconazole was at 0 ......
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