石决明清除1,1-二苯基-2-三硝基苯肼自由基活性的研究(1)
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[摘要] 目的：探讨石决明药效成分的抗氧化活性。方法：用CM-Sephadex C-25离子交换色谱(ion exchange chromatography，IEC)梯度层析分离石决明酸溶性提取物，得到各分离组分280 nm处光吸收曲线，SDS-PAGE方法分析各组分蛋白成分，最后测定了各组分的1，1-二苯基-2-三硝基苯肼自由基(DPPH·)清除活性。结果：IEC梯度层析得到双峰洗脱曲线，具有较高OD280 nm值的组分均显示出明显的SDS-PAGE蛋白电泳条带，DPPH·自由基清除能力实验结果表明，多个石决明IEC分离组分表现出良好的活性。结论：石决明除含有可能具有抗氧化活性的蛋白成分外，还存在可以清除DPPH·自由基的其他高活性物质。
[关键词] 石决明；离子交换色谱；蛋白；抗氧化
[中图分类号] R965 [文献标识码] C [文章编号] 1673-7210(2011)12(b)-005-03
Study on DPPH radical scavenging activity of Concha haliotidis
WANG Zhili1, LIANG Hongbao1, WU Jiulin1, CHEN Li1, ZHANG Qiqing1,2*
1.Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fujian Province, Fuzhou 350002, China; 2.Institute of Biomedical Engineering, Chinese Academy of Medical Sciences, Tianjin 300192, China
[Abstract] Objective: To evaluate antioxidant activity of Concha haliotidis. Methods: Acid-soluble extraction of Concha Haliotidis was applied to ion exchange chromatography (IEC) with a column of CM-Sephadex C-25, and then assayed by UV/Vis spectra at 280 nm. Fractions separated from Concha haliotidis solution by IEC were analyzed for protein content by SDS-PAGE method. Finally, DPPH radical scavenging ability of every constituent was determined. Results: The curve of IEC with grads elution exhibited two peaks and fractions with high absorption at 280 nm presented clear protein band in gel, the assay of DPPH radical scavenging ability showed that several fractions had stronger antioxidant activity than others. Conclusion: Concha haliotidis contains not only proteins with antioxidant potentials but also other components that can eliminate DPPH radicals.
[Key words] Concha haliotidis; Ion exchange chromatography; Protein; Antioxidant
石决明为海产鲍科软体动物鲍鱼(abalone)的贝壳，是一种名贵的动物类中药，具有平肝潜阳、清肝明目的功效。在水产养殖业，鲍鱼是一种重要的经济软体动物[1]，主要分布在东亚地区[2]，中国作为最大的鲍鱼生产国，每年要产生大量的鲍鱼壳，以致形成石决明药材产量过剩的局面。因此，有必要开展鲍鱼壳高附加值生物活性提取物方面的研究，填补该类中药开发研究领域的空白。
石决明同其他贝壳类物质一样，含有大量的碳酸钙、多种微量元素[4-5]和蛋白质成分等有机物。目前，对石决明的研究多集中在其成分、结构及两者的相关性方面，而对具有生物活性的药效成分的研究却鲜有报道。抗氧化作用是防治多种疾病的重要药效机制之一 ......
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