孕酮对视网膜缺血再灌注损伤神经元凋亡的影响(1)
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[摘要] 目的：研究孕酮预处理对视网膜缺血再灌注损伤后神经元凋亡的影响，并探讨其对视神经保护的可能机制。方法：用升高眼压的方法制作兔视网膜缺血再灌注模型，将36只新西兰大白兔随机分为正常组(n=4)、对照组(n=16)和孕酮组(n=16)。缺血再灌注模型制作前24 h，给予对照组兔耳缘静脉注射生理盐水4 ml/kg，孕酮组兔耳缘静脉注射孕酮4 mg/kg。观察缺血再灌注后不同时段对照组与孕酮组视网膜组织学变化和Bcl-2的蛋白表达及凋亡情况。结果：视网膜缺血再灌注各时间段，HE染色显示，孕酮组视网膜内层厚度均较对照组厚，且内核层细胞数较多，差异均有统计学意义(均P<0.05)；凋亡检测结果显示，缺血再灌注24 h时凋亡达高峰，孕酮组内核层的凋亡细胞少于对照组，差异有统计学意义 (P<0.05)； 孕酮组和对照组在缺血再灌注12 h时均检测到Bcl-2蛋白表达，孕酮组Bcl-2蛋白表达水平较对照组少(P<0.05)。结论：孕酮预处理可以促进视网膜缺血再灌注后细胞的存留，减少细胞凋亡，对视神经损伤具有保护作用。
[关键词] 孕酮；视网膜缺血；视神经损伤；凋亡；神经保护
[中图分类号] R774.105 [文献标识码] A [文章编号] 1673-7210(2011)12(b)-015-03
Influence of progesterone on neuronal apoptosis after retinal ischemia reperfusion injury
LEI Xiaoxi1, YAN Xiuju2, LIU Su3, WU Jun1, WANG Li1
1.Department of Ophthalmology, Nancheng People's Hospital of Dongguan City, Guangdong Province, Dongguan 523071, China; 2.Shiming Ophthalmology Center, the First People's Hospital of Chongqing City, Chongqing 400040, China; 3. Center of Ophthalmology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China
[Abstract] Objective: To study the influence of progesterone pretreatment on neuronal apoptosis after retinal ischemia reperfusion injury and investigate its potential protection mechanism for optic nerves. Methods: The intraocular pressure elevation method was used to build the rabbit retinal ischemia reperfusion model. 36 New Zealand white rabbits were randomly divided into normal group (n=4), control group (n=16) and progesterone group (n=16). 24 h before ischemia, the control group was treated with intravenous injection of normal saline 4 ml/kg in the ear margin, while the progesterone group was treated with 4 mg/kg of progesterone. The retinal histological changes, Bcl-2 protein expression and apoptosis of control group and protestation group in different time periods after ischemia reperfusion were observed ......
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