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丹红注射液预处理对大鼠缺血再灌注心肌细胞损伤的影响(1)
http://www.100md.com 2012年2月5日 郑亚萍 韩坤
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     [摘要] 目的 研究丹红注射液预处理对大鼠心肌细胞缺血再灌注损伤的作用。 方法 体外培养大鼠心肌细胞,将丹红注射液以2%、4%、8%、16%四种浓度分别加入培养基中,预处理24 h后,置于缺血再灌注(I/R)环境中培养,采用流式细胞仪技术检测各组细胞的凋亡率,免疫组织化学法检测凋亡基因Bax及Bcl-2蛋白表达变化。 结果 与正常对照组比较,缺血再灌组细胞凋亡率及Bas表达明显升高,Bcl-2表达则显著降低;而用丹红注射液预处理后呈浓度依赖性地下调凋亡率及Bas表达,上调Bcl-2表达。 结论 丹红注射液对缺血再灌心肌细胞有保护作用,且与浓度有一定的依赖关系,其作用可能是通过上调Bcl-2蛋白表达,抑制Bax蛋白表达实现的。

    [关键词] 丹红注射液;缺血再灌;心肌细胞;细胞凋亡

    [中图分类号] R331.3 [文献标识码] A [文章编号] 1673-7210(2012)02(a)-0024-02

    The effect of Danhong Injection pretreatment on cardiomyocytes cell of ischemic-reperfusion rats

    ZHENG Yaping HAN Kun

    Department of Physiology, Luohe Medical College, He′nan Province, Luohe 462002, China

    [Abstract] Objective To investigate the effect of Danhong Injection pretreatment on cardiomyocytes cell of ischemic-reperfusion rats. Methods The rats cardiomyocytes were cultured and pretreated with Danhong Injection in four different concentrations: 2%, 4%, 8%, 16% before create cardiomyocytes I/R model after 24 hours for pretreatment. The apoptotic rate of cardiomyocytes was determined by flow cytometry, the protein expressions of Bcl-2 and Bax were observed by imnmnohistochemistry. Results Compared with control group, the apoptotic rates were significantly increased in ischemia-reperfusion (I/R) group, and the protein expressions of Bax expressions was significantly up-regulated and Bcl-2 expressions was down-regulated. Pretreatment of cardiomyocytes with Danhong Injection increased the expression of Bcl-2, decreased the expression of Bax and the apoptotic rate in a dose-dependent fashion. Conclusion Danhong Injection alleviates I/R injury in a concentration-dependent manner. These effects may be partially that Danhong Injection can inhibit Bas gene protein expression and induce Bcl-2 gene protein expression.

    [Key words] Danhong Injection; Ischemia-reperfusion; Cardiomyocytes; Apoptosis

    心肌缺血再灌注损伤(isehemia-reperfusioninjury,I/R)是心脏缺血性疾病及心外术后心肌损害的主要原因,其导致的心肌细胞凋亡被认为是再灌注损伤的重要环节。丹红注射液是由丹参与红花萃取制备而成,具有保护心血管的作用,已有动物实验提示丹红注射液对大鼠离体心脏缺血再灌有保护作用[1],但其对缺血再灌心肌细胞的直接作用的报道较少。本研究采用心肌细胞缺血再灌注细胞模型,观察丹红注射液预处理对其的影响,初步研究其作用的可能机制。

    1 仪器与试药

    1.1 实验动物

    出生2~3 d的SD大鼠。

    1.2 试剂 ......

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