斑贞1号、川芎嗪和5-氟尿嘧啶联合对人胃癌细胞肺耐药蛋白基因表达影响(1)
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[摘要] 目的 探讨斑贞1号、川芎嗪(TMP)和5-氟尿嘧啶(5-FU)联合逆转人胃癌细胞系SGC-7901/ADR与LRP表达的相关性。 方法 采用半定量逆转录聚合酶链反应(RT-PCR)比较人胃癌细胞SGC-7901/ADR细胞在单纯5-FU和5-FU联合斑贞1号及TMP处理组肺耐药蛋白(LRP)mRNA的表达情况。 结果 5-FU组与阴性对照组比较，LRP mRNA表达差异无统计学意义(P > 0.05)；而5-FU联合斑贞1号及TMP处理组LRP mRNA表达量明显降低，差异有统计学意义(P < 0.05)。 结论 斑贞1号、TMP和5-FU联合逆转SGC-7901/ADR细胞耐药性可能与LRP基因相关，为当前胃癌的治疗提供了新的理论依据。
[关键词] 胃癌；斑贞1号；川芎嗪；5-氟尿嘧啶；多药耐药；LRP基因
[中图分类号] R735.2 [文献标识码] A [文章编号] 1673-7210(2012)04(a)-0029-02
Effect of Banzhen 1, Tetra methylpyrazine and 5 Fluorouracil on lung resistance protein of human gastric cancer cells gene expression
XUE Ximei1 FU Guizhen2 ZHANG Aimin3
1.Center of 18 Friendship Community, Baogang Hospital of Inner Mongolia, Inner Monggol Autonomous Region, Baotou 014030, China; 2.Huade County Hospital of Wulanchabu City, Inner Monggol Autonomous Region, Huade 013350, China; 3.Department of Gastroenterology, the First Affiliated Hospital of Baotou Medical College of Inner Mongolia University of Science and Technology, Inner Monggol Autonomous Region, Baotou 014010, China
[Abstracts] Objective To explore of the correlativity between Banzhen 1, Tetra methylpyrazine (TMP) and 5 Fluorouracil (5-Fu) reversing human gastric cancer cell line SGC-7901/ADR with lung resistance protein (LRP) expressions. Methods Semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) was used for comparison of LRP mRNA expression for cell line SGC-7901/ADR in group of 5-FU, 5-FU combined with Banzhen 1 and Tetramethylpyrazine (TMP). Results The expression of LRP mRNA between the 5-FU group and the control group, there was no significant difference (P > 0.05), while 5-FU and Banzhen 1 group and Tetra methylpyrazine (TMP) group was decreased significantly with significant difference (P < 0.05). Conclusion It is possible correlated to LRP for Banzhen 1, TMP and 5-FU reversed multidrug resistance in human gastric cancer cells, which may supply a new theoretical basis for the clinical effective treatment of gastric carcinoma.
[Key words] Stomach cancer; Banzhen 1; TMP; 5-FU; Multidrug resistance; LRP genes
化疗是治疗胃癌的有效手段之一，但胃癌多药耐药性(MDR)是导致化疗失败的主要原因。近几年发现肺耐药相关蛋白(lung resistance-related protein，LRP)在胃癌多药耐药性方面起着重要作用 ......
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