microRNA-34a与非小细胞肺癌的关系及其可能调节机制(1)
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[摘要] 目的 探讨miRNA-34a在非小细胞肺癌发生中的作用及其可能机制。 方法 选取56例经病理诊断确诊为非小细胞肺癌患者的癌组织及癌旁组织，RT-PCR法测定miRNA-34a及c-myc mRNA的表达水平，免疫组织化学法检测癌组织及癌旁组织的P53蛋白及c-myc蛋白的表达水平。 结果 癌组织的miRNA-34a的表达水平低于癌旁组织，c-myc mRNA表达及c-myc、P53蛋白表达阳性率高于癌旁组织(P < 0.05)。miRNA-34a与c-myc mRNA表达水平呈负相关关系(P < 0.05)。 结论 miRNA-34a对非小细胞肺癌的发生具有一定的调节作用，可能是非小细胞肺癌发生的重要环节，miRNA-34a主要是通过与c-myc的结合而发挥其调节作用。
[关键词] miRNA-34a；c-myc；P53；非小细胞肺癌
[中图分类号] R734.2 [文献标识码] A [文章编号] 1673-7210(2012)08(b)-0005-03
The relationship between microRNA-34a and non small-cell lung cancer and its possible regulation mechanism
ZENG Ting1 HAO Li1 XIE Yixin1 MA Li1 SHU Wenjie2
1.Center for Disease Control and Prevention in Guangzhou Military Region, Guangdong Province, Guangzhou 510507, China; 2.Academy of Military Sciences, Beijing 100080, China
[Abstract] Objective To explore the effect of miRNA-34a on the occurrence of small cell lung cancer (SCLC) and its possible mechanism. Methods Lung cancer tissues and its corresponding non cancer tissues in 56 cases of patients who diagnosed as SCLC by pathology were collected for detecting its miRNA and c-myc mRNA expression by RT-PCR. Immunohistochemistry method was used for detecting P53 and c-myc protein expression in cancer tissue and its corresponding non cancer tissues. Results Compared with corresponding non-cancer tissues, the expression of miRNA-34a in lung cancer tissue was lower, however the expression of c-myc mRNA, c-myc protein and P53 protein were higher (P < 0.05). There was a negative correlation between miRNA-34a and c-myc mRNA expression level (P < 0.05). Conclusion miRNA-34a plays a regulating role on the occurrence of SCLC, and it may be an important factor on SCLC occurrence by combination with c-myc ......
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