四种检测法在乙腈沉淀法去除血清蛋白中的比较(1)
 |
| 第1页 |
参见附件。
[摘要] 目的 比较四种方法在乙腈沉淀法去除血清蛋白中的检测效果，建立一种能快速检测去蛋白率的方法。 方法 用乙腈沉淀法去除血清蛋白，分别应用四种不同的检测分析方法，方法Ⅰ通过真空冻干；方法Ⅱ通过真空干燥；方法Ⅲ通过设立对照调零；方法Ⅳ通过挥干乙腈，分析血清蛋白的去除率。 结果 方法Ⅰ和方法Ⅱ耗时长、设备要求高；方法Ⅲ耗时最短、方法简便；方法Ⅳ耗时较短、但容易产生实验误差，方法Ⅰ、Ⅱ和Ⅲ的蛋白去除率结果比较，差异无统计学意义(P > 0.05)，与方法Ⅳ比较，差异有统计学意义(P < 0.05)。 结论 方法Ⅲ为最佳的分析测定分析方法，可用于快速方便了解乙腈的去蛋白率。
[关键词] 乙腈沉淀法；血清蛋白；去蛋白率
[中图分类号] R446.1 [文献标识码] A [文章编号] 1673-7210(2012)08(b)-0085-02
Comparison of four method for removing serum protein by acetonitrile precipitation
HU Honghua1 JIANG Ting1 SONG Haixing2▲ HU Handan1
1.School of Laboratory Medicine, Chengdu Medical College, Sichuan Province, Chengdu 610083, China; 2.Department of Biomedical Sciences, Chengdu Medical College, Sichuan Province, Chengdu 610083, China
[Abstract] Objective To compare the test effect of serum protein removal rate by acetonitrile precipitation with four different methods, the aim is to establish a rapid test method to detect the serum protein removal rate. Methods The serum proteins were removed by acetonitrile precipitation experiments, and applied for four different detection methods, vacuum freeze-drying (method Ⅰ), vacuum drying (method Ⅱ), controlled through the establishment of zero (method Ⅲ), dryness acetonitrile (method Ⅳ) were detected the serum protein removal rate in respect. Results Method Ⅰ and method Ⅱ were time-consuming and higher equipment requirements, while method Ⅲ was time-saving and simple, although method Ⅳ was time-saving, it was prone to experimental error, method Ⅰ, Ⅱ and Ⅲ proteins removal results revealed no significant difference (P > 0.05) compared with method Ⅳ, which had statistically significant difference (P < 0 ......
您现在查看是摘要介绍页，详见PDF附件(1967kb)。