脉冲磁疗对脑出血大鼠脑内谷氨酸和胰岛素样生长因子—1表达的影响(1)
[摘要] 目的 观察脉冲磁疗对脑出血大鼠脑组织中谷氨酸和胰岛素样生长因子-1(IGF-1)表达的影响。 方法 清洁级雄性SD大鼠36只，随机分成假手术组、模型组和磁疗组，每组又分为3 d组和7 d组，每个亚组各6只大鼠。采用立体定向技术制作脑出血模型。磁疗组于术后2 h开始脉冲磁疗干预，异名磁极对置于头部，磁距10 cm，平均磁场强度0.01 T，频率50 Hz，20 min/次，1 次/d。并于脑出血3、7 d时分别对各组大鼠进行神经行为学评定、谷氨酸和IGF-1浓度的测定。 结果 磁疗3、7 d组改良神经功能缺失评分(mNSS)均比对应的模型组下降，差异有高度统计学意义(P < 0.01)。脑出血后，脑组织中谷氨酸表达增加，经过脉冲磁疗3、7 d后，谷氨酸浓度均明显下降，低于模型组，差异有高度统计学意义(P < 0.01)。正常脑组织内IGF-1有少量表达，脑出血后，其表达增加；经过磁疗3、7 d后，与模型组相比，IGF-1水平均明显升高，差异有高度统计学意义(P < 0.01)。 结论 脉冲磁疗可以一定程度上促进脑出血大鼠神经功能的恢复，其机制可能与谷氨酸水平的下降和IGF-1的表达增加有关。
[关键词] 脉冲磁疗；脑出血；谷氨酸；胰岛素样生长因子-1
[中图分类号] R743 [文献标识码] A [文章编号] 1673-7210(2016)08(a)-0020-04
[Abstract] Objective To observe the effects of pulsed magnetic therapy on the expression of glutamate and insulin-like growth factor-l (IGF-1) after cerebral hemorrhage in rats. Methods 36 male adult Sprague-Dawley rats were randomly divided into the sham group (n=12)， model group (n=12) and magnetic therapy group (n=12). Each group was randomly divided into 3 d and 7 d subgroups， with 6 rats in each subgroup. The cerebral hemorhage model of rats was made using the technology of stereotaxis. The magnetic therapy group was treated with pulsed magnetic therapy (0.01 T， 50 Hz， 20 min/times， 1 times/d) after hemorrhage 2 h. The modified neurological severity score (mNSS)， the concentration of glutamate and IGF-1 were evaluated at 3 d and 7 d after cerebral hemorrhage. Results Compared to the model group， the mNSS scores in 3 d and 7 d magnetic therapy group decreased significantly (P < 0.01). After cerebral hemorrhage， the concentration of glutamate increased； in the magnetic therapy group， the concentration of glutamate was significantly decreased than in the model group after hemorrhage 3 d and 7 d (P < 0.01). IGF-1 had little expression in the normal brain tissue， after cerebral hemorrhage， its expression increased； the concentration of IGF-1 in the magnetic therapy group was significantly increased than in the model group after hemorrhage 3 d and 7 d (P < 0.01). Conclusion Pulsed magnetic therapy can promote the recovery of neural function in rats. This effect may be associated with the decline of glutamate and the higher expression of IGF-1.
[Key words] Pulsed magnetic therapy； Cerebral hemorrhage； Glutamate； Insulin like growth factor-l
脑出血后局部组织的水肿和继发性的神经细胞损伤是影响预后的重要因素。一方面，脑组织损伤的同时可以引起兴奋性氨基酸(excitatory amino acid，EAA)水平的升高，适量的EAA对维持神经细胞的兴奋性是必需的，但高浓度的EAA则具有神经毒性。谷氨酸作为EAA的一种，在中枢神经系统中含量最高。另一方面，脑组织损伤后，可引起内源性神经保护机制的启动，其中包括胰岛素样生长因子-1(insulin- like growth factor-1，IGF-1)表达的增加。脉冲磁疗是临床常用的物理治疗方法，许多研究都已经证实它可以促进脑损伤后神经功能的恢复，但对于其具体的作用机制，尚不十分清楚。本研究通过脉冲磁疗干预脑出血大鼠，探讨其对谷氨酸和IGF-1表达的影响，期望进一步明确磁疗在脑出血治疗方面的作用机制，为其临床应用提供进一步的理论依据。 (宋成宪 李舜 冯枫华 谢泽良 简颖敏)
[关键词] 脉冲磁疗；脑出血；谷氨酸；胰岛素样生长因子-1
[中图分类号] R743 [文献标识码] A [文章编号] 1673-7210(2016)08(a)-0020-04
[Abstract] Objective To observe the effects of pulsed magnetic therapy on the expression of glutamate and insulin-like growth factor-l (IGF-1) after cerebral hemorrhage in rats. Methods 36 male adult Sprague-Dawley rats were randomly divided into the sham group (n=12)， model group (n=12) and magnetic therapy group (n=12). Each group was randomly divided into 3 d and 7 d subgroups， with 6 rats in each subgroup. The cerebral hemorhage model of rats was made using the technology of stereotaxis. The magnetic therapy group was treated with pulsed magnetic therapy (0.01 T， 50 Hz， 20 min/times， 1 times/d) after hemorrhage 2 h. The modified neurological severity score (mNSS)， the concentration of glutamate and IGF-1 were evaluated at 3 d and 7 d after cerebral hemorrhage. Results Compared to the model group， the mNSS scores in 3 d and 7 d magnetic therapy group decreased significantly (P < 0.01). After cerebral hemorrhage， the concentration of glutamate increased； in the magnetic therapy group， the concentration of glutamate was significantly decreased than in the model group after hemorrhage 3 d and 7 d (P < 0.01). IGF-1 had little expression in the normal brain tissue， after cerebral hemorrhage， its expression increased； the concentration of IGF-1 in the magnetic therapy group was significantly increased than in the model group after hemorrhage 3 d and 7 d (P < 0.01). Conclusion Pulsed magnetic therapy can promote the recovery of neural function in rats. This effect may be associated with the decline of glutamate and the higher expression of IGF-1.
[Key words] Pulsed magnetic therapy； Cerebral hemorrhage； Glutamate； Insulin like growth factor-l
脑出血后局部组织的水肿和继发性的神经细胞损伤是影响预后的重要因素。一方面，脑组织损伤的同时可以引起兴奋性氨基酸(excitatory amino acid，EAA)水平的升高，适量的EAA对维持神经细胞的兴奋性是必需的，但高浓度的EAA则具有神经毒性。谷氨酸作为EAA的一种，在中枢神经系统中含量最高。另一方面，脑组织损伤后，可引起内源性神经保护机制的启动，其中包括胰岛素样生长因子-1(insulin- like growth factor-1，IGF-1)表达的增加。脉冲磁疗是临床常用的物理治疗方法，许多研究都已经证实它可以促进脑损伤后神经功能的恢复，但对于其具体的作用机制，尚不十分清楚。本研究通过脉冲磁疗干预脑出血大鼠，探讨其对谷氨酸和IGF-1表达的影响，期望进一步明确磁疗在脑出血治疗方面的作用机制，为其临床应用提供进一步的理论依据。 (宋成宪 李舜 冯枫华 谢泽良 简颖敏)