miR-15b在急性脑梗死患者血清中的表达及临床意义(1)
[摘要] 目的 探討miR-15b在急性脑梗死患者血清中的表达及临床意义。 方法 选取2018年1～12月广西中医药大学第一附属医院(以下简称“我院”)收治的98例急性脑梗死患者为急性脑梗死组，同期选取我院98例健康体检者为对照组。采用反转录-聚合酶链反应(RT-PCR)检测两组血清miR-15b表达;受试者工作特征曲线(ROC)评价miR-15b诊断急性脑梗死的价值;分析血清miR-15b表达与急性脑梗死临床病理特征的关系。结果 两组血清miR-15b分别为(1.28±0.38)、(2.33±0.72)，急性脑梗死组血清miR-15b表达高于对照组(P < 0.05)。miR-15b诊断急性脑梗死的ROC曲线下面积为0.817，95%CI = 0.721～1.438，敏感度为91.06%，特异度为79.35%。不同性别、年龄患者血清miR-15b表达差异无统计学意义(P > 0.05);不同梗死程度、梗死体积及预后患者血清miR-15b表达差异有统计学意义(P < 0.05)。 结论 miR-15b在急性脑梗死患者血清中呈高表达，且与梗死程度、梗死体积及预后可能有关，是诊断急性脑梗死的潜在血清学标志物。
[关键词] 急性脑梗死;miR-15b;诊断;预后
[中图分类号] R743.3 [文献标识码] A [文章编号] 1673-7210(2019)12(a)-0153-04
Expression and clinical significance of miR-15b in serum of patients with acute cerebral infarction
LI Sheng1 PAN Min1 GU Lian2 LI Shenfen1
1.Department of Laboratory Medicine， the First Affiliated Hospital of Guangxi University of Chinese Medicine， Guangxi Zhuang Autonomous Region， Nanning 530023， China; 2.Department of Encephalopathy， the First Affiliated Hospital of Guangxi University of Chinese Medicine， Guangxi Zhuang Autonomous Region， Nanning 530023， China
[Abstract] Objective To investigate the expression and clinical significance of serum miR-15b in patients with acute cerebral infarction. Methods From January to December 2018， 98 patients with acute cerebral infarction admitted to the First Affiliated Hospital of Guangxi University of Chinese Medicine (“our hospital” for short) were selected as the acute cerebral infarction group， during the same period， 98 healthy subjects in our hospital were as the control group. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expression of serum miR-15b in the two groups. The receiver operating characteristic curve (ROC) was used to evaluate the diagnostic value of miR-15b in acute cerebral infarction. The relationship between serum miR-15b expression and the clinicopathological characteristics of acute cerebral infarction was analyzed. Results Serum miR-15b in the two groups was (1.28±0.38) and (2.33±0.72)， respectively. The expression of serum miR-15b in acute cerebral infarction group was higher than that in control group (P < 0.05). The area under the ROC curve of miR-15b in diagnosing acute cerebral infarction was 0.817， 95%CI = 0.721-1.438， the sensitivity was 91.06%， and the specificity was 79.35%. There was no significant difference in serum mir-15b expression among patients of different genders and ages (P > 0.05). There were statistically significant differences in serum miR-15b expression in patients with different infarction degrees， infarction volumes and prognosis (P < 0.05). Conclusion MiR-15b is highly expressed in the serum of patients with acute cerebral infarction and may be related to the degree， volume and prognosis of infarction， which is a potential serological marker for the diagnosis of acute cerebral infarction., http://www.100md.com(李生盘 敏古联)
[关键词] 急性脑梗死;miR-15b;诊断;预后
[中图分类号] R743.3 [文献标识码] A [文章编号] 1673-7210(2019)12(a)-0153-04
Expression and clinical significance of miR-15b in serum of patients with acute cerebral infarction
LI Sheng1 PAN Min1 GU Lian2 LI Shenfen1
1.Department of Laboratory Medicine， the First Affiliated Hospital of Guangxi University of Chinese Medicine， Guangxi Zhuang Autonomous Region， Nanning 530023， China; 2.Department of Encephalopathy， the First Affiliated Hospital of Guangxi University of Chinese Medicine， Guangxi Zhuang Autonomous Region， Nanning 530023， China
[Abstract] Objective To investigate the expression and clinical significance of serum miR-15b in patients with acute cerebral infarction. Methods From January to December 2018， 98 patients with acute cerebral infarction admitted to the First Affiliated Hospital of Guangxi University of Chinese Medicine (“our hospital” for short) were selected as the acute cerebral infarction group， during the same period， 98 healthy subjects in our hospital were as the control group. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expression of serum miR-15b in the two groups. The receiver operating characteristic curve (ROC) was used to evaluate the diagnostic value of miR-15b in acute cerebral infarction. The relationship between serum miR-15b expression and the clinicopathological characteristics of acute cerebral infarction was analyzed. Results Serum miR-15b in the two groups was (1.28±0.38) and (2.33±0.72)， respectively. The expression of serum miR-15b in acute cerebral infarction group was higher than that in control group (P < 0.05). The area under the ROC curve of miR-15b in diagnosing acute cerebral infarction was 0.817， 95%CI = 0.721-1.438， the sensitivity was 91.06%， and the specificity was 79.35%. There was no significant difference in serum mir-15b expression among patients of different genders and ages (P > 0.05). There were statistically significant differences in serum miR-15b expression in patients with different infarction degrees， infarction volumes and prognosis (P < 0.05). Conclusion MiR-15b is highly expressed in the serum of patients with acute cerebral infarction and may be related to the degree， volume and prognosis of infarction， which is a potential serological marker for the diagnosis of acute cerebral infarction., http://www.100md.com(李生盘 敏古联)