基于UPLC指纹图谱的厚朴药材去粗皮质量评价研究(1)
[摘要] 目的 研究厚朴药材、去粗皮厚朴与厚朴粗皮主成分的含量和指纹图谱差异，为厚朴药材去粗皮入药提供依据。 方法 采用Waters Acquity UPLC BEH-C18色谱柱(50 mm×2.1 mm，1.7 μm)，以乙腈-0.2%磷酸溶液为流动相，梯度洗脱，流速0.4 mL/min，柱温35℃，检测波长230 nm。对不同产地厚朴药材、去粗皮厚朴与厚朴粗皮进行厚朴酚、和厚朴酚的含量测定，对比其共有峰的峰面积，并进行相似度分析。 结果 厚朴酚、和厚朴酚的总含量均为去粗皮厚朴>厚朴药材>厚朴粗皮;去粗皮厚朴6个共有峰的峰面积均比厚朴药材、厚朴粗皮的峰面积高。结论 厚朴去粗皮可提高厚朴饮片的质量，为厚朴去粗皮提供实验参考依据。
[关键词] 厚朴药材;去粗皮厚朴;厚朴粗皮;指纹图谱;相似度
[中图分类号] R282 [文献标识码] A [文章编号] 1673-7210(2020)07(a)-0027-04
[Abstract] Objective To study the content of main components and fingerprint differences of officinal magnolia bark， removing rough bark of officinal magnolia bark， rough bark of officinal magnolia bark， and provide evidence for the used as medicine of removing rough bark of officinal magnolia bark. Methods Waters Acquity UPLC BEH-C18 column (50 mm×2.1 mm， 1.7 μm) was used as the column. Acetonitrile-0.2% phosphoric acid solution was used as mobile phase for gradient elution. The flow rate was 0.4 mL/min， column temperature was 35℃， and the detection wavelength was 230 nm. The contents of magnolol and honokiol were determined for officinal magnolia bark from different areas， removing rough bark of officinal magnolia bark， rough bark of officinal magnolia bark. The peak area of the common peak was compared， and the similarity was analyzed. Results The total contents of magnolol and honokiol was removing rough bark of officinal magnolia bark>officinal magnolia bark>rough bark of officinal magnolia bark. The peak area of 6 common peaks of removing rough bark of officinal magnolia bark was higher than that of officinal magnolia bark and rough bark of officinal magnolia bark. Conclusion It can improve the quality of officinal magnolia bark pieces and provide experimental reference for removing rough bark of officinal magnolia bark.
[Key words] Officinal magnolia bark; Removing rough bark of officinal magnolia bark; Rough bark of officinal magnolia bark; Fingerprint; Similarity
厚樸始载于《神农本草经》[1]，为木兰科植物厚朴Officinal magnolia bark或凹叶厚朴Magnolia officinalis Rehd. et Wils. var. biloba Rehd. et Wils.的干燥干皮、根皮及枝皮。厚朴具有燥湿消痰、下气除满的功效[2]。厚朴产于湖北、四川等地，习称“川朴”，凹叶厚朴主要分布在浙江、福建、湖南南部等地，商品习称“温朴”[3]。相关文献报道[4-5]厚朴的主要化学成分有木脂素类、苯乙醇苷类、生物碱类以及挥发油等。现代药理证明，厚朴具有抗菌、抗炎、抗肿瘤、抗氧化等多种药理作用[6-7]。传统加工炮制过程中，皮类饮片多需除去外表粗皮，历代古籍皆有记载，如《本草纲目》记载“凡入药去粗皮”[8]。宋代《太平惠民和剂局方》亦云“凡使，先刮去粗皮，令见赤心”[9]，明代《医学入门》载“……如不去皮，耗人元气”[10]。《中华人民共和国药典》[11]2015年版一部厚朴饮片炮制项下也规定：厚朴需刮去粗皮。现代生产则简化工序，多不去皮。厚朴作为常用大宗药材，需求量很大，因此对厚朴饮片的质量控制尤为重要。目前，关于厚朴去粗皮化学成分的研究多集中在厚朴酚与和厚朴酚的含量变化上[12-14]，未见对其他成分影响的报道。本研究通过测定厚朴药材、去粗皮厚朴及厚朴粗皮的厚朴酚、和厚朴酚的含量与指纹图谱，对厚朴去粗皮前后成分进行进一步研究，为保证药效及用药的安全性，保证厚朴药用的科学合理性提供参考依据。, http://www.100md.com(李燕珍 鲁云 陈丹燕)
[关键词] 厚朴药材;去粗皮厚朴;厚朴粗皮;指纹图谱;相似度
[中图分类号] R282 [文献标识码] A [文章编号] 1673-7210(2020)07(a)-0027-04
[Abstract] Objective To study the content of main components and fingerprint differences of officinal magnolia bark， removing rough bark of officinal magnolia bark， rough bark of officinal magnolia bark， and provide evidence for the used as medicine of removing rough bark of officinal magnolia bark. Methods Waters Acquity UPLC BEH-C18 column (50 mm×2.1 mm， 1.7 μm) was used as the column. Acetonitrile-0.2% phosphoric acid solution was used as mobile phase for gradient elution. The flow rate was 0.4 mL/min， column temperature was 35℃， and the detection wavelength was 230 nm. The contents of magnolol and honokiol were determined for officinal magnolia bark from different areas， removing rough bark of officinal magnolia bark， rough bark of officinal magnolia bark. The peak area of the common peak was compared， and the similarity was analyzed. Results The total contents of magnolol and honokiol was removing rough bark of officinal magnolia bark>officinal magnolia bark>rough bark of officinal magnolia bark. The peak area of 6 common peaks of removing rough bark of officinal magnolia bark was higher than that of officinal magnolia bark and rough bark of officinal magnolia bark. Conclusion It can improve the quality of officinal magnolia bark pieces and provide experimental reference for removing rough bark of officinal magnolia bark.
[Key words] Officinal magnolia bark; Removing rough bark of officinal magnolia bark; Rough bark of officinal magnolia bark; Fingerprint; Similarity
厚樸始载于《神农本草经》[1]，为木兰科植物厚朴Officinal magnolia bark或凹叶厚朴Magnolia officinalis Rehd. et Wils. var. biloba Rehd. et Wils.的干燥干皮、根皮及枝皮。厚朴具有燥湿消痰、下气除满的功效[2]。厚朴产于湖北、四川等地，习称“川朴”，凹叶厚朴主要分布在浙江、福建、湖南南部等地，商品习称“温朴”[3]。相关文献报道[4-5]厚朴的主要化学成分有木脂素类、苯乙醇苷类、生物碱类以及挥发油等。现代药理证明，厚朴具有抗菌、抗炎、抗肿瘤、抗氧化等多种药理作用[6-7]。传统加工炮制过程中，皮类饮片多需除去外表粗皮，历代古籍皆有记载，如《本草纲目》记载“凡入药去粗皮”[8]。宋代《太平惠民和剂局方》亦云“凡使，先刮去粗皮，令见赤心”[9]，明代《医学入门》载“……如不去皮，耗人元气”[10]。《中华人民共和国药典》[11]2015年版一部厚朴饮片炮制项下也规定：厚朴需刮去粗皮。现代生产则简化工序，多不去皮。厚朴作为常用大宗药材，需求量很大，因此对厚朴饮片的质量控制尤为重要。目前，关于厚朴去粗皮化学成分的研究多集中在厚朴酚与和厚朴酚的含量变化上[12-14]，未见对其他成分影响的报道。本研究通过测定厚朴药材、去粗皮厚朴及厚朴粗皮的厚朴酚、和厚朴酚的含量与指纹图谱，对厚朴去粗皮前后成分进行进一步研究，为保证药效及用药的安全性，保证厚朴药用的科学合理性提供参考依据。, http://www.100md.com(李燕珍 鲁云 陈丹燕)