大豆黄素促脱落乳牙干细胞成骨的作用及其机制
1材料和方法,2结果,3讨论,4参考文献,人乳牙牙髓干细胞,成骨细胞分化
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[摘要]目的探讨大豆黄素促进脱落乳牙干细胞(SHED)成骨的作用及其机制。方法对体外培养的第3代SHED分别用100、10和1μmol·L-1的大豆黄素培养基进行培养，以普通培养基作为对照，于3、6、9 d检测其碱性磷酸酶(AKP)活性，于7、14、21 d检测其骨钙蛋白(OC)的质量，以RT-PCR检测其3、6、9 d的核心结合因子(cbf)-α1mRNA的表达。结果SHED经大豆黄素干预培养3 d，10μmol·L-1的大豆黄素显著提高了细胞内AKP的质量(与对照组比较，P<0.05)；培养6～9 d，3个浓度的大豆黄素均显著提高了AKP的表达量(与对照组比较，P<0.001)。中高浓度(10和100μmol·L-1)的大豆黄素可在成骨分化的中期(14 d)显著提高SHED内OC的质量(与对照组比较，P<0.001)，21 d时各浓度的大豆黄素均可促进OC的表达(与对照组比较，P<0.001)。SHED培养3 d，10和100μmol·L-1大豆黄素明显上调其cbf-α1mRNA的表达，1μmol·L-1的大豆黄素部分上调了cbf-α1 mRNA的表达；在第6和9天，1、10和100μmol·L-1的大豆黄素均促进了cbf-α1mRNA的表达，对照组在各时间点均呈阴性。结论大豆黄素可促进SHED向成骨方向分化，其机制可能与上调cbf-α1基因表达有关。
[关键词]大豆黄素；人乳牙牙髓干细胞；成骨细胞分化；机制
[中图分类号]Q 256[文献标志码]A[doi]10.3969/j.issn.1673-5749.2012.03.008
Effects and mechanism of daidzein on osteodifferentiation of stem cells derived from human exfoliated deciduous teethWu Bin1, Xie Chun1, Huang Qian2, Du Xinya1, Huang Jian1, Meng Qiuju1, You Yuehua1.(1. Center of Stomatology, Baoan Longhua Stomatological Hospital of Shenzhen, Shenzhen 518109, China; 2. Research Institute of Plastic Surgery, Guangzhou Medical College, Zhanjiang 524000, China)
[Abstract]ObjectiveTo investigate the effects and mechanism of daidzein on osteodifferentiation of stem cells derived from human exfoliated deciduous teeth(SHED). MethodsThe 3rdpassage SHED were incubated with 1, 10, 100μmol·L-1daidzein and normal culture medium(control). Then alkaline phosphatase(AKP)activities were examined at 3, 6 and 9 d, and osteocalcin(OC)in each group was detected at 7, 14 and 21 d. In addition, corebinding factor(cbf)-α1mRNA expression were detected by RT-PCR at 3, 6 and 9 d after culture. Results3 d later, AKP activities in SHED were enhanced by 10μmol·L-1daidzein(vs. control, P<0.05), all of three concentrations(1, 10, 100μmol·L-1)daidzein improved AKP activities significantly at 6 and 9 d(vs. control, P<0.001). Medium and high dose(10, 100μmol·L-1)daidzein could enhance OC expression in SHED at 14 d after induced culture(vs. control, P<0.001), and all of the doses daidzein increased OC expression at 21 d(vs ......
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