胃动素对大鼠下丘脑胃牵张敏感神经元和胃运动的影响(英文)
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赵一方 徐珞 青岛大学医学院病理生理学教研室;
【摘要】目的:研究胃动素对下丘脑弓状核胃牵张敏感神经元放电活动和胃运动的影响。方法:采用4管玻璃微电极细胞外记录胃动素对大鼠弓状核胃牵张敏感神经元活动,采用胃内置传感器观察胃动素对对清醒大鼠胃运动的影响。结果:65.5%的弓状核神经元为胃扩张敏感性神经元,其中55.6%为胃扩张兴奋性神经元,44.4%为抑制性神经元。胃扩张刺激后兴奋性神经元的放电频率显著增加(P0.01),而抑制性神经元的放电频率显著降低(P0.01)。弓状核内微量注射胃动素,70%的兴奋性神经元在胃扩张刺激后表现为兴奋作用,17.5%的神经元表现为抑制作用,并且放电频率显著增加(P0.05)。同样,在抑制性神经元中,65.6%在注射胃动素后引起电活动增强,放电频率显著降低(P0.05)。而胃动素受体拮抗剂GM-109可以完全阻断这种由胃动素诱导的兴奋作用,提示,胃动素在弓状核通过其特异性受体调控神经元活动。在胃运动实验中,弓状核微量注射胃动素后,胃运动的收缩频率和幅度都显著增加(P0.05);同时,这种兴奋作用也可被GM-109阻断。结论:研究证实了弓状核胃动素神经元接收来自胃感受器的外周躯体感觉传入神经的冲动,并通过某些下级核团通路发挥胃运动的调节作用。
【关键词】 胃动素 弓状核 GM- 胃扩张敏感神经元 胃运动
【基金】National Natural Science Foundation of China(No.30470642and No.30670780) Shandong Province Tackle Key Problems in Science and Technology Program(2008GG10002006) Qingdao Municipal Scienceand Technology Commission(05-1-JC-93) Shandong province Health Department 266021(2007-37)~~
【分类号】Q4
IntroductionMotilin is a member of the‘gut-brain’family of peptidehormones.It is secreted by Mo endocrine cells located in themucosa of the stomach and intestine as well as by specializedneurons in the myenteric plexus and brain[1-4],participates incon
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Introduction
Motilin is a member of the 'gut-brain' family of peptide hormones. It is secreted by Mo endocrine cells located in the mucosa of the stomach and intestine as well as by specialized neurons in the myenteric plexus and brain, participates in controlling the pattern of smooth muscle contractions in the upper gastrointestinal tract and stimulate gastrointestinal motility. Recently, a series of the central nervous system (CNS) effects of motilin on gastrointestinal activity has been studies. It showed that motilin could significantly enhance gastric motility by administration of motilin in the lateral hypothalamus (LH), ventral medial hypothalamus (VMH)and amygdala. And administration of erythromycin, a motilin receptor agonist, could stimulate the gastric motility in diabetic rats. Furthermore, lateral ventricle microinjeeted motilin could change the firing frequency of gastric distention related neurons in dorsal vagal complex(DVC), LH and hippocampus.It is known that Arc is a key hypothalamic nucleus in the regulation of appetite. However, up to now an involvement of Arc motilin in the regulation of digestive functions has not been demonstrated. This paper was (1) to investigate the influence of the central motilin on the spontaneous electrical activity of such neurons, (2) to explore the effect of administration of motilin on gastric motility.
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