健脾活血方对大鼠24 h急性酒精中毒的保肝效应及机制研究(1)
摘要 目的：研究健脾活血方對大鼠24 h急性酒精中毒的保肝效应及作用机制。方法：28只SD大鼠随机分为正常组(8只)、模型组和健脾活血方组(各10只)。予相应饮用水或健脾活血汤1.0 mL/100 gwt，连续3 d，2次/d。第3天末次给药后，禁食禁水16 h，再次灌胃饮用水或健脾活血汤1.0 mL/100 gwt。1 h后，予等剂量56%乙醇灌胃模型组和健脾活血方组大鼠复制酒精中毒模型。24 h后麻醉，留取血清及肝组织标本，检测相关指标。结果：与模型组比较，健脾活血方组血清ALT活性显著降低，差异有统计学意义( P <0.01)HE染色见肝细胞胞质内小空泡状改变减轻，库普弗细胞减少，肝组织GSH活性显著升高( P <0.01)，肝组织TNOS、iNOS活性显著降低( P <0.05)。结论：健脾活血方通过提高24 h急性酒精中毒大鼠肝脏GSH含量，降低iNOS水平，起到抗氧化应激损伤保护肝脏的作用。
关键词 健脾活血方；急性酒精中毒；氧化应激；肝损伤
Study on Mechanism of Hepatoprotective Effect of Jianpihuoxue Fang on 24 Hours Acute Alcohol Intoxication Rats
Xu Lin1，3，4， Feng Qin2，3，4， Peng Jinghua2，3，4， Fu Qilin2， Huang Fu2， LI Xiaofei2， Zhao Yu2，3，4， Hu Yiyang2，3，4，5
(1 Institute of Kidney Diseases， Department of nephrology， Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine， Shanghai 201203， China； 2 Institute of Liver Diseases， Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine， Shanghai 201203， China； 3 Shanghai Key Laboratory of Traditional Chinese Clinical Medicine (14DZ2273200)， Shanghai 201203， China； 4 Key Laboratory of Liver and Kidney Diseases， Ministry of Education， Shanghai University of Traditional Chinese Medicine， Shanghai 201203， China； 5 E-institute of Traditional Chinese Internal Medicine of Shanghai Municipal Education Commission， Shanghai 201203， China)
Abstract Objective： To explore the mechanism of hepatoprotective effect of Jianpihuoxue Decoction on 24 hours acute alcohol intoxication rats and to discuss its mechanism of anti-oxidative stress. Methods： Twenty-eight Sprague Dawley rats were allocated to the normal group (8 rats)， acute alcoholism model group (10 rats) and the jianpihuoxue decoction group (10 rats). Each group was given the jianpihuoxue decoction or water 1.0 mL/100 gwt twice a day for three days. The water and food were forbidden for 16 hours after the last administration three days later.， then rats were given jianpihuoxue decoction or water 1.0 mL/100 gwt by gavage again.One hour later， acute alcoholism model group and the jianpihuoxue decoction group were given the same amount of 56% alcoholl 1.5 mL/100 gwt by gavage to reproduce the model of acute alcoholism. The serum and hepatic tissues were taken after 24 h to detected the related indexes. Results： Compared with the model group， Jianpihuoxue decoction reduced activities of serum ALT( P <0.01)， alleviated microvesicular steatosis obviously and kupffer cells in the hepatocytes reduced， observed by H&E staining. The content of GSH of liver tissue increased significantly ( P <0.01) while the expression of TNOS and iNOS lowered ( P <0.05). Conclusion： Jianpihuoxue decoction protects the 24 hours acute alcoholism rats′ livers and resists the oxidative stress damage by increasing the level of hepatic GSH， decreasing the level of hepatic iNOS., 百拇医药(徐琳 冯琴 彭景华 傅琪琳 黄甫 李晓飞 赵瑜 胡义扬)
关键词 健脾活血方；急性酒精中毒；氧化应激；肝损伤
Study on Mechanism of Hepatoprotective Effect of Jianpihuoxue Fang on 24 Hours Acute Alcohol Intoxication Rats
Xu Lin1，3，4， Feng Qin2，3，4， Peng Jinghua2，3，4， Fu Qilin2， Huang Fu2， LI Xiaofei2， Zhao Yu2，3，4， Hu Yiyang2，3，4，5
(1 Institute of Kidney Diseases， Department of nephrology， Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine， Shanghai 201203， China； 2 Institute of Liver Diseases， Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine， Shanghai 201203， China； 3 Shanghai Key Laboratory of Traditional Chinese Clinical Medicine (14DZ2273200)， Shanghai 201203， China； 4 Key Laboratory of Liver and Kidney Diseases， Ministry of Education， Shanghai University of Traditional Chinese Medicine， Shanghai 201203， China； 5 E-institute of Traditional Chinese Internal Medicine of Shanghai Municipal Education Commission， Shanghai 201203， China)
Abstract Objective： To explore the mechanism of hepatoprotective effect of Jianpihuoxue Decoction on 24 hours acute alcohol intoxication rats and to discuss its mechanism of anti-oxidative stress. Methods： Twenty-eight Sprague Dawley rats were allocated to the normal group (8 rats)， acute alcoholism model group (10 rats) and the jianpihuoxue decoction group (10 rats). Each group was given the jianpihuoxue decoction or water 1.0 mL/100 gwt twice a day for three days. The water and food were forbidden for 16 hours after the last administration three days later.， then rats were given jianpihuoxue decoction or water 1.0 mL/100 gwt by gavage again.One hour later， acute alcoholism model group and the jianpihuoxue decoction group were given the same amount of 56% alcoholl 1.5 mL/100 gwt by gavage to reproduce the model of acute alcoholism. The serum and hepatic tissues were taken after 24 h to detected the related indexes. Results： Compared with the model group， Jianpihuoxue decoction reduced activities of serum ALT( P <0.01)， alleviated microvesicular steatosis obviously and kupffer cells in the hepatocytes reduced， observed by H&E staining. The content of GSH of liver tissue increased significantly ( P <0.01) while the expression of TNOS and iNOS lowered ( P <0.05). Conclusion： Jianpihuoxue decoction protects the 24 hours acute alcoholism rats′ livers and resists the oxidative stress damage by increasing the level of hepatic GSH， decreasing the level of hepatic iNOS., 百拇医药(徐琳 冯琴 彭景华 傅琪琳 黄甫 李晓飞 赵瑜 胡义扬)
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