蓝莓花青素对人胃腺癌BGC-823细胞增殖、侵袭的影响及机制研究(1)
摘要 目的：探讨蓝莓花青素(BA)对人胃腺癌BGC-823细胞增殖、侵袭的影响及相关机制。方法：低、中、高剂量实验组与对照组人胃腺癌BGC-823细胞分别以25、50、100 μg/mL BA与等量生理盐水处理，分别处理12 h、24 h、48 h。采用噻唑蓝(MTT)与流式细胞仪检测人胃腺癌BGC-823细胞增殖与凋亡;采用Transwell小室实验与免疫细胞化学法检测人胃腺癌BGC-823细胞侵袭与P53、半胱氨酸蛋白酶-3(Caspase-3)蛋白表达。结果：干预48 h时，对照组、低、中、高剂量实验组人胃腺癌BGC-823细胞P53与Caspase-3蛋白阳性细胞百分比分别为(11.12±1.01)%，(24.31±2.18)%，(47.56±2.91)%，(66.49±5.14)%与(15.04±1.23)%，(28.22±3.09)%，(69.36±5.42)%，(86.52±6.15)%。实验组胃腺癌BGC-823细胞增殖抑制率随BA浓度增大而逐渐升高;与对照组比较，低、中、高剂量实验组人胃腺癌BGC-823细胞凋亡率、P53与Caspase-3蛋白阳性细胞百分比均显著升高，侵袭率降低，呈浓度依赖性(均P<0.05)。结论：BA可有效抑制胃腺癌BGC-823细胞增殖，诱导其凋亡，并降低侵袭能力，其机制可能与上调P53与Caspase-3蛋白表达有关。
关键词 胃腺癌;蓝莓花青素;增殖;侵袭;P53;半胱氨酸蛋白酶-3
Abstract Objective：To investigate the effects and related mechanism of blueberry anthocyanins (BA) on the proliferation and invasion of human gastric adenocarcinoma BGC-823 cells.Methods：Human gastric adenocarcinoma BGC-823 cells exp-L/M/H and the control group were treated with BA at the concentration of 25，50，100 μg/mL and the same amount of normal saline respectively，and each group was treated for 12，24，and 48 hours.The proliferation and apoptosis of human gastric adenocarcinoma BGC-823 cells was detected by MTT assay and Flow cytometry; Transwell chamber assay and immunocytochemistry method were used to detect the invasion of human gastric adenocarcinoma BGC-823 cells and the expression of P53 and caspase-3 protein.Results：At 48 h after intervention，the percentage of P53 cells and Caspase-3 protein positive cells in human gastric adenocarcinoma BGC-823 cells in the exp-L/M/H were (11.12±1.01)%，(24.31±2.18)%，(47.56±2.91)%，(66.49±5.14)% and (15.04±1.23)%，(28.22±3.09)%，(69.36±5.42)%，and (86.52±6.15)%，respectively.With the increase of BA concentration，the proliferation inhibition rate of gastric adenocarcinoma BGC-823 cells in experimental group increased gradually; Compared with the control group，the apoptotic rate，P53 protein and Caspase-3 protein positive percentage of human gastric adenocarcinoma BGC-823 cells in the exp-L/M/H groups significantly increased，while the invasive rate decreased and the trend was concentration-dependent (all P<0.05).Conclusion：BA can effectively inhibit the proliferation of gastric adenocarcinoma BGC-823 cells，induce its apoptosis，and reduce its invasive ability，which may be related to the up-regulation of P53 protein and Caspase-3 protein expression.
Keywords Gastric adenocarcinoma; Blueberry anthocyanins; Proliferation; Invasion; P53; Cysteine protease-3
胃癌是較为常见的恶性肿瘤，具有高度异质性，侵袭和转移力极强[1]。已有研究证实，花青素可抑制肺癌、结肠癌及乳腺癌等多种肿瘤细胞增殖及侵袭[2]。研究发现，蓝莓花青素(BA)可通过线粒体凋亡途径对人胃腺癌BGC-823细胞具有生长抑制和诱导凋亡作用[3]。也有研究发现，BA可通过下调P53基因DNA甲基化抑制口腔癌KB细胞增殖及诱导凋亡[4]。目前，关于BA在胃腺癌细胞方面的相关研究报道较少，因此本研究旨在探讨BA对人胃腺癌BGC-823细胞增殖、侵袭能力及P53、半胱氨酸蛋白酶-3(Caspase-3)表达的影响，为临床治疗胃腺癌寻找高效、低毒的干预新手段。, 百拇医药(艾福录 吕武 赵国华)
关键词 胃腺癌;蓝莓花青素;增殖;侵袭;P53;半胱氨酸蛋白酶-3
Abstract Objective：To investigate the effects and related mechanism of blueberry anthocyanins (BA) on the proliferation and invasion of human gastric adenocarcinoma BGC-823 cells.Methods：Human gastric adenocarcinoma BGC-823 cells exp-L/M/H and the control group were treated with BA at the concentration of 25，50，100 μg/mL and the same amount of normal saline respectively，and each group was treated for 12，24，and 48 hours.The proliferation and apoptosis of human gastric adenocarcinoma BGC-823 cells was detected by MTT assay and Flow cytometry; Transwell chamber assay and immunocytochemistry method were used to detect the invasion of human gastric adenocarcinoma BGC-823 cells and the expression of P53 and caspase-3 protein.Results：At 48 h after intervention，the percentage of P53 cells and Caspase-3 protein positive cells in human gastric adenocarcinoma BGC-823 cells in the exp-L/M/H were (11.12±1.01)%，(24.31±2.18)%，(47.56±2.91)%，(66.49±5.14)% and (15.04±1.23)%，(28.22±3.09)%，(69.36±5.42)%，and (86.52±6.15)%，respectively.With the increase of BA concentration，the proliferation inhibition rate of gastric adenocarcinoma BGC-823 cells in experimental group increased gradually; Compared with the control group，the apoptotic rate，P53 protein and Caspase-3 protein positive percentage of human gastric adenocarcinoma BGC-823 cells in the exp-L/M/H groups significantly increased，while the invasive rate decreased and the trend was concentration-dependent (all P<0.05).Conclusion：BA can effectively inhibit the proliferation of gastric adenocarcinoma BGC-823 cells，induce its apoptosis，and reduce its invasive ability，which may be related to the up-regulation of P53 protein and Caspase-3 protein expression.
Keywords Gastric adenocarcinoma; Blueberry anthocyanins; Proliferation; Invasion; P53; Cysteine protease-3
胃癌是較为常见的恶性肿瘤，具有高度异质性，侵袭和转移力极强[1]。已有研究证实，花青素可抑制肺癌、结肠癌及乳腺癌等多种肿瘤细胞增殖及侵袭[2]。研究发现，蓝莓花青素(BA)可通过线粒体凋亡途径对人胃腺癌BGC-823细胞具有生长抑制和诱导凋亡作用[3]。也有研究发现，BA可通过下调P53基因DNA甲基化抑制口腔癌KB细胞增殖及诱导凋亡[4]。目前，关于BA在胃腺癌细胞方面的相关研究报道较少，因此本研究旨在探讨BA对人胃腺癌BGC-823细胞增殖、侵袭能力及P53、半胱氨酸蛋白酶-3(Caspase-3)表达的影响，为临床治疗胃腺癌寻找高效、低毒的干预新手段。, 百拇医药(艾福录 吕武 赵国华)