姜婷婷 江宇泳 叶永安

    摘要 目的：探讨健脾解毒凉血方治疗肝损伤肠屏障功能障碍的作用靶点。方法：选取C57BL/6雄性小鼠32只，随机分为正常组、模型组、中药组、培菲康组，腹腔注射硫代乙酰胺100 mg/kg造模;中药组予以健脾解毒凉血方灌胃，培菲康组给予培菲康溶液灌胃;16 h后采集小鼠血清、肝组织、小肠组织，观察小鼠肝功能、肠道组织HE染色变化，免疫组织化学染色方法观察小肠闭锁小带蛋白-1表达。结果：模型组、中药组、培菲康组小鼠的ALT、AST均显著高于正常组，模型组、中药组、培菲康组比较差异无统计学意义(P>0.05)。小肠组织在光镜和电镜下均可观察到中药组病变轻于模型组和培菲康组，免疫组织化学染色在小肠上皮细胞上可见闭锁小带蛋白-1棕黄色阳性标记，模型组、中药组、培菲康组阳性标记均显著少于正常组，中药组阳性标记显著多于模型组(P0.05).There was no significant difference between the model group，the Chinese medicine group and the Peifikang group.Small intestinal tissue can be observed under light and electron microscopy.Immunohistochemical staining in the cell membrane of intestinal villus epithelial cells were visible on the zonula occludens protein-1 Brown positive marker.The positive markers of model group，Chinese medicine group and Peifikang group were significantly less than which of normal group.The positive marker of Chinese medicine group were significantly more than that of model group(P22 g予以270 μL灌胃、体质量75%阳性信号 ......