王风波+唐明薇+董海

    [摘要] 目的 探讨电针对脑出血大鼠出血同侧海马区Nogo-A表达的影响及作用机制。 方法 将66只成年SD大鼠按随机数字表法分为对照组和脑出血组，脑出血组再随机分为非干预组和电针组，Ⅶ型胶原酶脑内注射诱导大鼠尾状核出血，电针组于造模次日开始电针干预，各组大鼠于第2、5、10、15天取出血侧海马C1区脑组织石蜡切片行Nogo-A免疫组化检测，每例脑组织取3张切片，每张切片于400倍光镜下随机选取5个互不叠加视野计数阳性细胞并取其平均值。 结果 脑出血组大鼠右侧尾状核区域有血肿形成；与对照组比较，非干预组与电针组海马区Nogo-A阳性表达水平均显著增强(P < 0.01)，而电针组较非干预组表达水平明显下降(P < 0.01)。 结论 电针干预可显著抑制脑出血大鼠脑内Nogo-A的表达，有利于脑出血后中枢神经轴突的再生修复，促进神经功能恢复。

    [关键词] 电针；海马区；脑出血；Nogo-A

    [中图分类号] R245.97 [文献标识码] A [文章编号] 1674-4721(2016)10(c)-0037-04

    [Abstract] Objective To investigate the effects and mechanism of electro-axupuncture on the expression of Nogo-A in hippocampus at the same side of bleeding in rats with cerebral hemorrhage. Methods Sixty-six adult SD rats were randomly divided into control group and cerebral hemorrhage group by the random number table， and the cerebral hemorrhage group was randomly divided into non-intervention group and electro-axupuncture group. Hemorrhage of caudate nucleus in rats was induced by intracerebral injection of collagenase Ⅶ ......