星形胶质细胞在完全弗氏佐剂诱导的炎性痛中的作用(1)
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【摘要】 目的 探讨脊髓背角星形胶质细胞在完全弗氏佐剂(complete Freunds adjuvant，CFA)诱导的炎性痛中的作用。方法 雄性SD大鼠随机分为3组(6只/组)：CFA组(大鼠左后肢足底注射CFA诱导炎性痛，同时行鞘内置管术，术后6 d鞘内给予生理盐水)；Lαaminoadipate(LAA)组(处理方式同CFA组，但术后6 d鞘内给予LAA)；对照组(处理方式同CFA组，但足底注射生理盐水)。术前2 d及术后7 d检测各组机械痛和热痛阈值，术后7 d以Realtime PCR法检测脊髓背角GFAP mRNA变化。结果 术前2 d各组大鼠左后肢足底机械痛及热痛阈值无明显差异(P> 0.05)。术后7 d，CFA组大鼠脊髓背角GFAP mRNA较对照组明显增高(P< 0.01)，左后肢足底机械痛及热痛阈值较对照组明显降低(P< 0.01)；与CFA组相比，LAA组大鼠脊髓背角GFAP mRNA明显降低(P< 0.01)，左后肢足底机械痛及热痛阈值均明显增高(P< 0.01)。结论 LAA特异性抑制脊髓背角星形胶质细胞活性，缓解了CFA大鼠炎性痛，提示脊髓背角星形胶质细胞活化是CFA诱导炎性痛的重要机制。
【关键词】
完全弗氏佐剂；炎性痛；星形胶质细胞
Role for astrocytes in complete freunds adjuvant (CFA) induced inflammatory pain
ZHANG Tao, CHEN Lei, ZHANG Yiliang, et al.Department of Neurology, Hospital of Beijing Armed Police Corps, Beijing 100027, China
【Abstract】 Objective
To investigate the role for astrocytes in CFAinduced inflammatory pain. Methods Male SD rats were assigned into 3 groups (n = 6/group): CFA group (intraplantar injection of CFA into the hindpaw of rats to induce inflammatory pain, and intrathecal implanted for administration with normal saline 6 d after operation), Lαaminoadipate (LAA) group (as CFA group treated except for intrathecally administration with LAA), control group (as CFA group treated except for intraplantar injection of CFA). Mechanical allodynia and thermal hyperalgesia were observed 2 d preoperation and 7 d postoperation. Furthermore, 7 days after operation, alterations of glial fibrillary acidic protein (GFAP) in spinal dorsal horn were detected at mRNA level. Results There was no difference of paw withdrawal threshold (PWL) or thermal response latencies (TRL) within groups 2 d preoperation (P> 0.05). Seven days postoperation, PWL and TRL were significantly decreased, and spinal dorsal horn GFAP mRNA was increased in CFA group compared with control group (P< 0.01). While, PWL and TRL were significantly increased, and spinal dorsal horn GFAP mRNA was decreased in LAA group compared with CFA group 7 d postoperation (P< 0 ......
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