前S1抗原在乙型肝炎诊断中的应用研究(1)
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【摘要】 目的 研究在不同病情乙肝患者血清中前S1抗原(HBVpre-S1)水平的变化与HBV血清标志物、HBV-DNA的临床相关性,评价HBVpre-S1的检测在乙型肝炎诊断中的应用价值。方法 用酶联免疫吸附法(ELISA)分别对268例HBV血清进行五项血清标志物,Pre-S1的检测。用全自动生化分析仪赖氏法检测肝功能,同时采用PCR荧光定量法检测HBV-DNA的量。结果 196例慢性乙肝(CH)患者中,HBeAg(+)组和HBeAg(-)组比较,Pre-S1(+)和HBVDNA(+)检出率及Pre-S1/HBsAg的相对比值均高于HBeAg(-)组,差异具有统计学意义P<0.01;且在HBeAg(-)组中。HBeAb(-)组和HBeAb(+)组相比,Pre-S1/HBsAg相对比值也显著高于HBeAb(+)组,P<0.05;在CH患者中,轻、中、重三组间的Pre-S1(+)率,HBVDNA及Pre-S1/HBsAg相对比值相比较,差异均无统计学意义,且Pre-S1/HBsAg相对比值水平和肝脏炎症程度无关。在72例AH患者中,Pre-S1/HBsAg的相对比值与肝脏急性损伤的严重程度相关,在AH患者Pre-S1/HBsAg相对比值水平下降越早越快,病程越短,预后越好。结论 Pre-S1可作为HBV复制的标志物;而Pre-S1/HBsAg的相对比值的高低,在肝炎的诊断,AH预后的判断方面具有重要的价值;联合检测Pre-S1可补充和完善HBV五项检测的不足,尤其对HBeAg(-)或者变异的HBV感染者能更好地反映病毒的复制状态和传染性。
【关键词】 乙肝病毒;前S1抗原;比值比;HBV-DNA;临床实验室技术
Hepatitis B pre-S1 antigen in the Diagnosis
LI Jing-peng.
Department of Nuclear Medicine,First People’s Hospital of shangqiu,Henan 476100,China
【Abstract】 Objective To study the serum of patients with different disease Hepatitis B antigen pre-S1(HBVpre-S1)levels in patients with HBV serum markers,HBV-DNA of the clinical relevance,evaluation HBVpre-S1 the detection of hepatitis diagnosis value.Methods Used enzyme linked immunosorbent assay(ELISA)were conducted on 268 cases of the five serum HBV serum markers,Pre-S1 test.Automatic biochemical analyzer Lai liver function was detected,and detected by fluorescence quantitative PCR HBV-DNA quantity.Results 196 patients with chronic hepatitis B(CH)patients,HBeAg(+)group,and HBeAg(-)group,Pre-S1(+)and HBVDNA(+)detection rate and the relative ratio of both high Pre-S1/HBsAg in HBeAg(-)group,the difference was significant,P<0.01;and in HBeAg(-)group.HBeAb(-)group and HBeAb(+)group compared to,Pre-S1/HBsAg relative ratio was also significantly higher than HBeAb(+)group,P<0.05.In CH patients,mild,moderate and severe among the three groups of Pre-S1(+)rate,HBVDNA and Pre-S1/HBsAg relative ratio,the difference was not statistically significant,and the relative ratio Pre-S1/HBsAg levels and liver inflammation grade ......
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