大鼠肾缺血再灌注对Smad7表达的影响(1)
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[摘要]目的探讨大鼠肾缺血再灌注后不同时间点Smad7的表达，寻找临床预测急性肾功能衰竭的新指标及治疗的新手段。方法建立大鼠肾缺血再灌注模型，当再灌注达相应时间点时，采用全自动生化分析仪、免疫组化、HE染色分别测定或观察血肌酐、尿素氮、肾组织Smad7表达、肾组织病理改变。结果①肾功能改变：与假手术组比较，0h、4h、12h、24h时模型组BUN及Cr均高于假手术组(P<0.01)。②肾脏组织学改变：模型组的肾组织损伤较假手术组明显加重。③肾组织Smad7的表达：模型组与同一时间点假手术组比较，在0h时肾脏Smad7阳性表达两组表达无差异(P>0.05)；在4h、12h、24h模型组肾脏Smad7阳性表达较假手术组均减少(P均<0.01)。 结论 随着肾功能逐渐恶化的同时Smad7表达不断减少。
[关键词] Smad7表达；肾缺血再灌注损伤；血肌酐；血尿素氮
[中图分类号] R692.5 [文献标识码] A [文章编号] 1673-9701(2011)20-06-03
Influence of Smad7 Expression in Renal Ischemia-Reperfusion Injury of Rats
XU JunnanWANGRongrongWANG Zhiyi WANG ZhengGONG YuqiangSUN LaifangCHEN Daqing
Department of Emergency Medicine，the Second Affiliated Hospital of Wenzhou University，Wenzhou 325027，China
[Abstract] ObjectiveTo investigate Smad7’s expression in different time in renal ischemia-reperfusion injury(IRI)and search new clinical index and method to forecast acute renal failure. Methods Establishing the model of renal ischemia-reperfusion， When reperfused to the corresponding time point， blood samples were obtained for measurement of serum levels of creatinine(Cr) and blood urea nitrogen(BUN).Renal tissue was used for histological examination and immuohistochemical analysis of Smad7. Results①Renal function change： Compared with the control group at the time point of 4h，12h，24h after reperfusion， the serum levels of BUN and Cr in the subgroup of the model group were significantly higher(P<0.01)；②Renal cellular damages in the model group showed more severe than those in the control group； ③Expression of Smad7 in kidney of 0h reperfusion were not different statistically in two groups (P>0.05). Expression of Smad7 in kidney of the model group were lower significantly in 4h，12h，24h reperfusion than the control group(P<0.01). ConclusionWith the gradual deterioration of renal function，the expression of Smad7 is decreased.
[Keywords] Smad7 expression；Ischemia-reperfusion injury； Creatinine； Blood urea nitrogen
肾缺血再灌注损伤(ischemia-reperfusion injury，IRI)是指肾脏缺血组织在重新获得血供后，组织细胞损伤未见减轻反而加重的一种病理现象。TGF-β/Smads蛋白通路是肾脏非常重要的一条信号转导途径，Smad7是TGF-β/Smads蛋白信号通路中最重要的内源性抑制因子，是Smads蛋白家族中的重要一员。前人对此通路作了大量的研究，作为引起急性肾衰肾前性因素的缺血再灌注在此方面研究较少，为了弄清楚肾缺血再灌注时Smad7表达是增加[1]还是减少 ......
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