酒石酸布托啡诺后处理对离体大鼠心肌缺血再灌注损伤的影响(1)
 |
| 第1页 |
参见附件(2902KB，3页)。
[摘要] 目的 探讨酒石酸布托啡诺后处理对大鼠离体心肌缺血再灌注损伤的影响。方法 应用Langendorff离体灌流装置，采用完全停灌复灌的方法制作离体大鼠心肌缺血再灌注模型。健康雄性SD大鼠32只，体重220～250g，随机分为4组：对照组(Sham组，n=8)、缺血再灌注组(I/R组，n=8)、缺血预处理组(pre组，n=8)和酒石酸布托啡诺后处理组(butor组，n=8)。制备大鼠离体心脏缺血再灌注损伤模型，于再灌注结束后，收集冠脉流出液测定肌酸激酶(CK)和乳酸脱氢酶(LDH)的活性，测定心肌组织丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性。结果 与Sham组比较，其他各组冠脉流出液CK和LDH活性及心肌组织MDA含量升高，心肌组织SOD活性降低(P<0.01)；与I/R组比较，pre组及butor组冠脉流出液CK和LDH活性及心肌组织MDA含量降低，心肌组织SOD活性升高(P<0.01)，而pre组与butor组比较，CK和LDH活性及心肌组织MDA含量和SOD活性差异无统计学意义(P>0.05)。结论 酒石酸布托啡诺后处理对离体心脏缺血再灌注损伤具有保护作用。
[关键词] 酒石酸布托啡诺；后处理；心肌缺血再灌注损伤
[中图分类号] R541 [文献标识码] A [文章编号] 1673-9701(2011)20-18-03
The Effects of Butorphanol of Postconditioning on Myocardial Ischemia Reperfusion Injury in Isolated Rat Hearts
YAN Xuetao1，2ZHENG Wenzhong1WU Yun2WANG Yanlin2
1.Department of Anesthesiology，Shenzhen Baoan Maternity and Child Health Hospital，Shenzhen 518133，China；2.Department of Anesthesiology，Zhongnan Hospital of Wuhan University，Wuhan 430071，China
[Abstract] Objective To explore the effects of postconditioning of butorphanol on myocardial ischemia/reperfusion(I/R) injury in isolated rat hearts. MethodsThirty-two male SD rats weighing 220-250kg were randomly divided into 4 groups： sham operation group(Sham group，n=8)，the isolated rat hearts were perfused with an oxygenated(95%O2-5%CO2)K-H solution at 37°C in a Langendorff apparatus for 120min； I/R group(IR group，n=8)，the isolated rat hearts were perfused with K-H solution for 30min then reperfusion for 40min after 50min of stabilization；Ischemia preconditioning group(pre group，n=8)，the isolated rat hearts were with 3 times transient 3min in 5min out perfusion before stopping irrigation for 20min，then reperfusion for 50min. Butorphanol postconditioning group(butor group，n=8)，which were subjected to 30min of global ischemia followed by 50min of reperfusion after 20min of stabilizationand and isolated hearts were perfused with 1mol L-1 butorphanol for 30min before ischemia ......
您现在查看是摘要介绍页，详见PDF附件(2902KB，3页)。