缬沙坦与氟伐他汀降低阿霉素肾病大鼠蛋白尿及肾组织NF-κB表达实验研究
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2011年7月25日
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[摘要] 目的 探讨缬沙坦与氟伐他汀对阿霉素(ADR)肾病大鼠蛋白尿及肾组织核因子κB(NF-κB)表达的影响。方法 雄性SD大鼠120只，适应性喂养2周后，随机抽取18只为正常对照组(A组)；另外102只制作阿霉素肾病模型。84只造模成功大鼠随机分为4组：B组为模型对照组(等容积生理盐水， n=21)，C组为缬沙坦治疗组[缬沙坦3.5mg/(kg·d)，n=21]，D组为氟伐他汀治疗组[氟伐他汀10mg/(kg·d)，n=21]，E组为缬沙坦与氟伐他汀联合治疗组[缬沙坦3.5mg/(kg·d) 加氟伐他汀10mg/(kg·d)，n=21]。其后，分别于2、6、10周末，遵循随机化原则，按n=6分层抽取各组样本，收集24h尿液、血液及肾组织标本待测。结果 与A组相比,B组、C组、D组和E组24h尿蛋白排泄、血清总胆固醇(TC)、甘油三酯(TG)浓度明显升高(P<0.01)；免疫组化染色表明， NF-κB阳性积分明显升高(P<0.01)；缬沙坦与氟伐他汀单用及联用尿蛋白排泄减少，血清TC、TG浓度降低(P<0.05 或 P<0.01)，肾脏NF-κB表达降低(P<0.05 或 P<0.01)。结论 缬沙坦与氟伐他汀可减轻阿霉素肾病大鼠蛋白尿、降低血清TG、TC浓度，降低肾脏NF-κB表达，联用时疗效更明显。提示缬沙坦与氟伐他汀至少部分通过降低NF-κB表达而减轻肾脏损害。
[关键词] 阿霉素肾病；缬沙坦；氟伐他汀；核因子κB
[中图分类号] R587.2[文献标识码] A[文章编号]1673-9701(2011)21-06-03
Effect of Valsartan and Fluvastatin on Reducting the Expression of Proteinuria and Nuclear Factor-κB (NF-κB) in Adriamycin-induced Nephrotic Rats
ZHANG DaoyouWU XiaodongCUI MingchunYANG YanlangYANG LicaiZHU Xinjian.
Departnent of Nephrology, Affiliated Yijishan Hospital,Wannan Medical College,Wuhu 241001,China
[Abstract] Objective To investigate the influence effct of valsartan and fluvastatin on reducting the expression of proteinuria and nuclear factor -κB(NF-κB)in adriamycin-induced nephrotic rats. Methods Male Sprague-Dawley(SD)rats were randomly separated into five groups and given different therapies. Normal control group(group A,n=18). Nephrotic model was induced by tail introvenously injection of ADR(6.0mg/kg). Eighty-four ADR-induced nephrotic male SD rats were randomly divided into 4 groups: model control group(group B: normal saline 3mL/d,n=21), valsartan treatment group(group C, valsartan 3.5mg/(kg·d), n=21),fluvastatin treatment group(group D, fluvastatin 10mg/(kg·d), n=21) and combining valsartan with fluvastatin treatment group(group E,valsartan 3 ......
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