丹参酚酸B后处理脱细胞猪心脏瓣膜的形态学及生物力学研究(1)
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[摘要]目的 探讨曲拉通法联合丹参酚酸B处理对猪心脏瓣膜形态学及生物力学的影响，为组织工程提供理论支持。方法 新鲜的猪主动脉瓣叶，采用两种不同方法脱细胞处理，应用光镜、电镜进行形态学观察，厚度仪测量组织厚度，行拉力测试观察生物力学的差异。结果 两种方法处理后组织厚度差异无统计学意义(P>0.05)，曲拉通-丹参酚酸B法较好地保留心脏瓣膜原有的三维空间，且生物力学测试优于传统曲拉通法(P<0.05)。结论 曲拉通联合丹参酚酸B处理可以制备基质成分保留充分、生物力学性能优良的心脏瓣膜支架，显示了可观的临床应用前景。
[关键词] 丹参酚酸B；后处理；组织工程；心脏瓣膜；形态学；生物力学
[中图分类号] R318.08 [文献标识码] A [文章编号]1673-9701(2011)22-13- 02
Effect of Salviaolic Acid B Postconditioning on Morphology and Biomechanics in Decellularized Porcine Aortic Valve
TAO Rancen GUO Guangwei
Department of Cardiothorocic Surgery，the Second Affiliated Hospital of Shanxi Medical University，Taiyuan 030001，China
[Abstract] Objective To observe the effect of salviaolic acid B postconditioning on morphology and biomechanics of decellularized pocine aortic valve，providing theoretical support for tissue engineering. Methods Porcine aortic valve leaflets were theated by two different proticols. Control group1 involved treatment with Triton-x100，sodium deoxycholate，DNase and RNase. And group 2 were treated by Triton-x100，sodium deoxycholate，DNase and RNase， then using salviaolic acid B post-processing. Eath samples were observered by microscopy and scanning electron microscope； Biomechanics characteristics measured by biomechanics properties-maximum deflection，elongation rate and max tensile stress. Statistical analysis was performed to compare the thickness. Results There was no significant difference in tissue thickness was found between two methods，Tx-sal method can protect the heart valve three-dimensional structure，biomechanics properties-maximum deflection ， max load and elongation rate were increased significantly in the group with salviaolic acid B. Conclusion Salviaolic acid B post-processing can effectively protect the collagen and elastic fibers and improve the biomechanical properties of decellularized pocine aortic valve.
[Key words] Salviaolic acid B； Postconditioning；Tissue engineering；Heart valves； Morphology；Biomechanics
理想的组织工程异种生物瓣膜支架应彻底去除细胞成分、最大限度地保存弹性纤维和胶原纤维，才能最大程度地减低免疫原性，较好地保留细胞黏附所需的三维微环境，进而更好地避免在瓣膜置换术后瓣膜的钙化和腐败 ......
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