新城疫病毒作用下卵巢癌裸鼠移植瘤Survivin、Caspase-3的表达(1)
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[摘要] 目的 研究新城疫病毒(Newcastle disease virus，NDV)对卵巢癌裸鼠移植瘤Survivin、Caspase-3表达的影响。方法 ①细胞培养：人卵巢癌细胞SKOV-3细胞的培养；②建立裸鼠移植瘤模型；③实验分组：分为实验组与对照组，实验组为肿瘤内注射NDV病毒抗卵巢癌； ④应用免疫组化SP法观察Survivin、Caspase-3的表达。结果 Survivin表达实验组与对照组均为阳性，实验组明显弱于对照组，差异有显著性(P<0.05) ；Caspase-3表达实验组均为阳性，对照组1例表达，实验组明显强于对照组，差异有显著性(P<0.05)。结论NDV可诱导肿瘤细胞凋亡，抑制其生长；其机制可能通过激活Caspase-3凋亡，抑制Survivin抗调亡作用。
[关键词]卵巢癌；NDV；裸鼠移植瘤；免疫组化
[中图分类号] R737.31 [文献标识码] A [文章编号]1673-9701(2011) 24-06-02
The Expression of Survivin and Caspase-3 in the Ovarian Cancer Nude Mice Transplanted Tumor by Newcastle Disease Virus
SU Lirong
Department of Obstetrics and Gynecology，Beijing Municipal Shunyi District Health Care Hospital for Women and Children， Beijing 101300， China
[Abstract] Objective To investigate the expression of Survivin and Caspase-3 in the ovarian cancer nude mice injected by Newcastle disease virus. Methods SKOV-3 tumor cells were cultured；the model of SKOV-3 transplanted tumor model in the nude mice were established. We injected the NDV into the transplanted tumor in experiment group，water was injected in control group；Immunohistochemistry SP was used to detect the expression of Survivin and Caspase-3. Results The expression of Survivin was positive in both A and B group and the stain result in group A was lighter than in group B(P<0.05). The expressions of Caspase-3 were all positive in experiment group，only had one in control group and experiment group was inferior than control group(P<0.05).Conclusion When NDV is injected into，apoptosis is induced and the growth of neoplastic cell is inhibited. The mechanisms may be through activation of Caspase-3 and inhibition of anti-apoptosis effect of Survivin.
[Key words] Ovarian cancer；NDV；Transplanted tumor in nude mice；Immunohistochemistry
卵巢恶性肿瘤严重威胁妇科肿瘤患者生命，生物治疗作为肿瘤治疗的模式日益受到重视。新城疫病毒(Newcastle disease virus，NDV)是近年引起重视的一种病毒，有一定抑瘤作用。肿瘤的发生发展与细胞凋亡异常关系密切，凋亡的调控中凋亡抑制蛋白起重要作用。凋亡抑制蛋白Survivin通过抑制凋亡蛋白Caspase-3的活化或抑制己活化的蛋白酶活性发挥作用。Caspase-3是细胞凋亡调控中的关键蛋白酶，Survivin的抑制凋亡功能最强。
本实验研究NDV对卵巢癌裸鼠移植瘤细胞Survivin、Caspase-3表达的影响，进一步证实NDV可诱导肿瘤细胞凋亡，抑制其生长，其机制可能通过激活Caspase-3凋亡，抑制Survivin抗调亡作用。
1 材料与方法 ......
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