δ阿片受体激动剂DADLE对脓毒症大鼠血清HMGB1表达的影响及其作用的研究(1)
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[摘要] 目的 观察δ阿片受体激动剂DADLE对脓毒症大鼠血清HMGB1水平及器官功能的影响。 方法 96只SD大鼠分为假手术组(SO)、脓毒症组(SEP)、DADLE组[制模后腹腔内注射DADLE(5 mg/kg)]，各32只。盲肠结扎穿孔(CLP)建立脓毒症模型，制模后6、12、18、24 h各取8只处死取材。ELISA法检测血清HMGB1水平及24 h ALT、AST、BUN、Cr、CK及PaO2的变化。 结果 HMGB1水平在SEP组、DADLE组12 h 18 h及24 h均较SO组高(P < 0.05)，DADLE组则显著低于SEP组(P < 0.05)。24 h时DADLE组血ALT、AST、BUN、Cr、CK均较SEP组明显改善，PaO2明显升高(P < 0.05)，且各指标变化与HMGB1有显著相关性(P < 0.05)。 结论DADLE抑制HMGB1释放，对脓毒症大鼠重要脏器功能有保护作用。
[关键词] DADLE；脓毒症；高迁移率族蛋白1；器官功能
[中图分类号] R459.7 [文献标识码] A [文章编号] 1673-9701(2012)30-0004-02
Study on the effect of delta-opioid receptor agonist on serum HMGB1 in sepsis rat
CHAI Zhenzhong TANG Chengwu FENG Wenming BAO Ying ZHU Ming
Department of Hepatobiliary Surgery and Molecular Surgery，the First People’s Hospital Affiliated to Huzhou University Medical College， Huzhou 313000，China
[Abstract] Objective To observe the effects of delta-opioid receptor agonist(DADLE) treatment on the expression of serum HMGB1 and multiple organ function in sepsis rat. Methods A total of 96 SD rats were randomly divided into sham operated group(SO)，septic group(SEP) and DADLE group(DADLE). Septic model was produced by cecel ligation and puncture(CLP). In SO group，the abdomen was opened without any other treatment. In DADLE group，DADLE was administered at a dose of 5 mL/kg by intra-peritoneal injection after CLP. Rats were sacrificed at 6 h，12 h，18 h and 24 h after CLP. HMGB1 level was determined by ELISA，and organ functional parameters were collected. Results Compared with sham operation group，the serum HMGB1 increased in DADLE treatment group and sepsis group(P < 0.05) at 12 h， 18 h and 24 h after CLP，the result in DADLE treatment markedly decreased than that in sepsis group(P < 0 ......
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