不同提取工艺下甘草提取物中甘草酸铵含量测定研究(1)
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[摘要] 目的：建立甘草提取物中甘草酸铵的含量测定方法，优选甘草酸铵的提取工艺。方法：以甘草酸铵为指标，采用高效液相色谱法测定甘草酸铵含量；比较煎煮法、水提醇沉法、0.5%氨水提取与70%乙醇超声法等工艺的差异。结果：0.5%氨水提取甘草酸铵含量较高，甘草酸铵含量在5.267 5～337.12 μg范围里呈较好的线性关系；甘草酸铵的回收率为98.51%。结论：0.5%氨水提取工艺所得甘草提取物中甘草酸铵含量较高，甘草酸铵转移率最高，提取方法合理、可行、效率高。
[关键词] 甘草提取物；甘草酸铵；提取工艺；高效液相色谱法
[中图分类号] R931 [文献标识码] C [文章编号] 1674-4721(2011)11(c)-047-03
Study on content determination of Monoammonium Glycyrrhizinate in the distilled licorice by different extraction technique
HUANG Huilian, ZHOU Yanshuang, SONG Lili
Guangzhou Wanglaoji Pharmaceutical Company Limited, Guangdong Province, Guangzhou 510450, China
[Abstract] Objective: To establish a method for content determination of Monoammonium Glycyrrhizinate in the distilled licorice and to optimize the extraction technique. Methods: Taking Monoammonium Glycyrrhizinate as index, we used the technique of HPLC to determine its content. Then compared the difference among water-decocting water-extracting and alcohol-depositing; 0.5% ammonia-extracting and 70% alcohol ultrasonic-method. Results: The content from 0.5% ammonia-extracting extraction of Monoammonium Glycyrrhizinate was the higher. In the range of 5.267 5-337.12 μg, the Monoammonium Glycyrrhizinate showed good linear relationship. The recovery of Monoammonium Glycyrrhizinate was 98.51%. Conclusion: The content from 0.5% ammonia-extracting extraction of Monoammonium Glycyrrhizinate is the higher. The transfer rate of Monoammonium Glycyrrhizinate is the highest. The 0.5% ammonia-extracting is reasonable, feasible and efficient.
[Key words] The distilled licorice; Monoammonium Glycyrrhizinate; Extraction technique; HPLC
甘草为豆科植物甘草(Glycyrrhiza uralensis Fisch)、胀果甘草(Glycyrrhiza inflata Bat)或光果甘草(Glycyrrhiza glabra L)的干燥根及根茎。具有补脾益气，清热解毒，祛痰止咳，缓急止痛，调和诸药的作用。主要用于脾胃虚弱，倦怠乏力，心悸气短，咳嗽痰多，脘腹、四肢挛急疼痛，痈肿疮毒，缓解药物毒性、烈性[1]。在中成药生产中，常以甘草的提取物入药。其主要活性成分为甘草酸铵(glycyrrhizic acid，GA)，对许多疾病有预防和治疗作用。甘草酸铵也是甘草甜味的有效成分，它的特点是高甜度、低热能、安全无毒，起泡性和溶血性作用低。能与多种生物碱、抗生素、氨基酸等生成复盐或复方制剂，具有协同、增溶、增加药物稳定性、提高生物利用率及降低毒副作用的功效。同时，还广泛用于食品添加剂、化妆品、烟草行业[2]。我公司小儿七星茶颗粒成方制法中的甘草也是以提取物入药，它的提取工艺与纯化方法直接影响该产品的质量及溶化性。因此，研究甘草提取物的提取工艺及纯化方法具有重要意义。本实验通过优选甘草的提取工艺 ......
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