通脉丸中竹节香附素A及总皂苷含量测定(1)
 |
| 第1页 |
参见附件。
[摘要] 目的：建立通脉丸中竹节香附素A及总皂苷含量测定方法。方法：采用HPLC法测定竹节香附素A的含量，色谱条件：AgilentSB-AQ色谱柱，乙腈与0.1%磷酸水为流动相梯度洗脱，流速0.8 ml/min，检测波长206 nm；采用比色法，以竹节香附素A为对照品，5%香草醛-冰乙酸-60%硫酸为显色剂，在531 nm处测定吸光度，计算总皂苷的含量。结果：液相法测定竹节香附素A对照品质量在0.573～3.820 μg(R2=0.999 6)范围内呈良好线性关系，平均加样回收率为99.63%(RSD=1.05%)；比色法测定竹节香附素A对照品质量在0.024 2～0.217 8 mg范围内与吸光度呈良好线性关系(R2=0.999 0)，平均加样回收率为100.05%(RSD=1.69%)。结论：建立了通脉丸中竹节香附素A及总皂苷含量测定方法，此方法简便、准确、稳定，为其制剂的质量控制及新制剂的二次开发提供技术支持。
[关键词] 通脉丸；竹节香附素A；总皂苷；含量测定
[中图分类号] R286.0 [文献标识码] A [文章编号] 1674-4721(2012)01(a)-005-03
The determination of Raddeanin A and total saponins in Tongmai pill
CHEN Tianchao1, WANG Junjie2
1. Department of Pharmacy, The First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Henan Province, Zhengzhou 450000, China; 2. Pharmaceutical College, Henan Collenge of Traditional Chinese Medicine, Henan Province, Zhengzhou 450008, China
[Abstract] Objective: To establish a method for the determination of Raddeanin A and total saponins in Tongmai pill. Methods: A HPLC determination of Raddeanin A, Chromatographic conditions: Agilent SB-AQ chromatographic column, Gradient elution by Acetonitrile and 0.1% phosphoric acid water as mobile phase,velocity 0.8 ml/min, wavelength was 206 nm; Colorimetric method used to Raddeanin A as reference, 5% vanillin-acetic acid-60% sulfuric acid as the color reagent, absorbance was measured at 531 nm to calculate the total saponin content. Results: Determination of liquid Reference standard of Raddeanin A quality was linear in the range of 0.573-3.820 μg (R2=0.999 6). The average recovery was 99.63% (RSD=1.05%); Colorimetric method determination Raddeanin A quality was linear in the range of 0.024 2-0.217 8 mg (R2=0.999 6). The average recovery was 100.05% (RSD =1.69%). Conclusion: Established Tongmai pill of Raddeanin A and total saponins method for the determination , this method is simple, accurate, stable, for quality control and preparation of the secondary development of new agents to provide technical support ......
您现在查看是摘要介绍页，详见PDF附件(2204kb)。