一氧化氮对大鼠心脏骤停复苏后脑组织作用研究(1)
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[摘要] 目的 探讨一氧化氮(NO)对大鼠心脏骤停(SCA)复苏后脑组织的损伤作用。 方法 90只SD大鼠(雌雄不限)随机分为3组：假手术(A)组、NS对照(B)组、iNOS抑制剂干预(C)组，每组均分为6、12、24、48、72 h时间点，每时间点6只；除A组外，B、C组均于复苏即刻给药。制作窒息致大鼠SCA模型，自主循环恢复(ROSC)后各时间点取脑组织检测NO及脑组织含水量。 结果 (1)脑组织NO含量：A组各时间点之间比较，差异无统计学意义(P > 0.05)；B、C组与A组比较浓度明显增加，差异具有统计学意义(P < 0.01)；C组较B组各时间点比较结果降低，差异具有统计学意义(P < 0.05)。(2)脑组织含水量：A组各时间点之间比较，差异无统计学意义(P > 0.05)；B、C组与A组比较含水量明显增加，差异具有统计学意义(P < 0.01)；C组较B组各时间点比较差异有统计学意义(P < 0.05)。 结论 窒息致大鼠SCA复苏成功6 h后脑组织NO含量上升，24 h达高峰，与脑水肿程度基本一致，给予诱导型一氧化氮合成酶(iNOS)抑制剂能抑制大鼠脑组织NO表达，减轻脑水肿，说明病理条件下产生的NO对复苏后脑组织起损伤作用。
[关键词] 一氧化氮；心脏骤停；脑水肿；实验研究
[中图分类号] R541.7+8 [文献标识码] A [文章编号] 1674-4721(2012)02(c)-0005-03
Nitric oxide on rats brain tissue after cardiopulmonary resuscitation
TAN Weihua ZHOU Yuan GUO Wenlong NIE Wei JIANG Qixin QING Chen
ICU of The First Hospital of Hunan Traditional Chinese Medical College in Hunan Province, Zhuzhou 412000, China
[Abstract] Objective To investigate effect of the nitric oxide on brain tissue after cardiac arrest (SCA) of the rats. Methods Ninty SD rats were divided into three groups without sex consideration: sham operation group (group A), NS control group (group B), and iNOS inhibitor intervention group (group C), and according to 6, 12, 24, 48, 72 h time point, each group were divided into five subgroups with 6 rats in each time point; group A were only given organ intubation and femoral vein catheter, group B and group C were made to cardiac arrest or asphyxia cardiac arrest model on the basis of group A, group C were given rapid intravenous injection of iNOS immediately after resusciation with on repetition later in order to build SCA model induced by asphyxia, and rats were sacrificed to detected brain water content as well as the expression of NO in rat brain. Results (1) NO content of brain tissue: Group A had a lower expression than group B and C with significant difference(P < 0 ......
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