蛇床子素逆转人膀胱肿瘤T24/ADM细胞耐药作用及其机制(1)
 |
| 第1页 |
参见附件(2248KB，3页)。
[摘要] 目的 研究蛇床子素(osthole，OST)对T24/ADM 细胞多药耐药的逆转及对P-gp表达、功能的影响。 方法 采用MTT法筛选OST对T24/ADM 细胞无毒或低毒浓度，以筛选出的浓度作为耐药逆转的实验浓度。分别利用MTT法分别检测阿霉素(adriamycin，ADM)、氟尿嘧啶(fluorouracil，FU)及顺铂(cisplatin，DDP)对细胞株T24和T24/ADM以及OST处理后的T24/ADM细胞的半数抑制浓度IC50。用流式细胞仪测定OST对T24/ADM 细胞上P-gp 表达的影响。 结果 浓度17 μmol/L OST对T24/ADM无明显细胞毒性，T24/ADM对ADM、FU和DDP均有不同程度的。耐药，耐药倍数分别为18.86、5.09、3.28，使用17 μmol/L OST处理后T24/ADM对上述ADM、FU和DDP的敏感性均有不同程度的提高，ADM对耐药细胞株的IC50由(0.962±0.017) μg/mL 降至(0.364±0.031) μg/mL，逆转指数为2.64；FU对耐药细胞株的IC50由(0.723±0.003) μg/mL降至(0.463±0.021) μg/mL，逆转指数为1.56；DDP对耐药细胞株的IC50由(0.664±0.007) μg/mL降至(0.587±0.016) μg/mL, 逆转指数为1.13，使T24/ADM细胞内P-gp 蛋白表达明显下降，下降了16.32%(P < 0.05)。 结论 蛇床子素在体外能部分逆转T24/ADM的耐药性，逆转机制可能是通过抑制P-gp蛋白表达实现的。
[关键词] 蛇床子素；T24/ADM细胞；耐药逆转；机制
[中图分类号] R737.1 [文献标识码] A [文章编号] 1674-4721(2012)03(a)-0007-03
Reversal effect and mechanism of osthole on the multidrug-resistance of human bladder cancer cells T24/ADM
WANG Xiaohua1 ZHANG Shaojin1 GUO Xiang1 SUN Mengliang1 LI Qinpeng1 LIU Hongyao2
1.Shanxi Medical University, Taiyuan 030001, China；2.Department of Urology, Shanxi Da Hospital, Shanxi Academy of Medical Sciences, Taiyuan 030001, China
[Abstract] Objective To study the reversal of osthole on the role of drug resistance T24/ADM cells. Methods The low or no cytotoxicity concentration of OST for T24/ADM cells by MTT, and take these concentrations as the experimental concentrations. The inhibition rate of ADM, FU, DDP without and with no cytotoxicity concentration of OST to T24 and T24/ADM cells were evaluated With MTT assay. The IC50 of different agents was counted. The concentrations of P-gp in T24/ADM cells with and without OST were measured by flow cytometey(FCM). Results Osthol at the concentration of 17 μmol/L had no significant cytoxicity to T24/ADM cells. T24/ADM cells were resistant to ADM, FU and DDP and the resistance index was 18.86, 5.09, 3.28 respectively. The sensitivity of T24/ADM to ADM, FU and DDP was significantly increased by osthol in the concentration of 20 μmol/L. The inhibitive concentration 50 ( IC50) of ADM declined from (0 ......
您现在查看是摘要介绍页，详见PDF附件(2248KB，3页)。