斑贞1号、川芎嗪和氟尿嘧啶联合对人胃癌细胞多药耐药基因1表达的影响(1)
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[摘要] 目的 探讨斑贞1号、川芎嗪(TMP)和氟尿嘧啶(5-FU)联合逆转人胃癌细胞系SGC-7901/ADR与多药耐药基因1(MDR1)表达的相关性。 方法 以SGC-7901/ADR细胞为靶细胞，以不加药组为阴性对照组，氟尿嘧啶组、斑贞1号组、斑贞1号+氟尿嘧啶组、斑贞1号+氟尿嘧啶+川芎嗪(TMP)组为实验组，采用半定量RT-PCR法检测SGC-7901/ADR细胞在不同药物作用下MDR1 mRNA的表达情况。 结果 氟尿嘧啶组与阴性对照组相比，MDR1 mRNA表达差异无统计学意义(P > 0.05)，而其他药物组与对照组及组间比较差异有统计学意义(P < 0.05)，斑贞1号+氟尿嘧啶 + TMP组表达量最少。 结论 斑贞1号、TMP和氟尿嘧啶联合逆转SGC-7901/ADR细胞耐药性可能与MDR1基因相关，为当前胃癌的治疗提供了新的理论依据。
[关键词] 胃癌；斑贞1号；川芎嗪(TMP)；氟尿嘧啶；多药耐药；多药耐药基因1(MDR1)
[中图分类号] R735.2 [文献标识码] A [文章编号] 1674-4721(2012)03(a)-0061-02
Effect of Ban Zhen 1, tetramethylpyrazine and fluorouracil on human gastric cancer cells multidrug resistance gene expression
FU Guizhen1 ZHANG Aimin2
1.Huade County Hospital of Wulanchabu City in Inner Mongolia Autonomous Region, Huade 013350, China; 2.Department of Gastroenterology, the First Affiliated Hospital of Baotou Medical College, Baotou 014010, China
[Abstract] Objective To explore the correlativity of Ban Zhen 1, Tetramethylpyrazine (TMP) and fluorouracil reversing human gastric cancer cell line SGC-7901/ADR with Multidrug resistance gene 1 (MDR1) expressions. Methods SGC-7901/ADR cells was considered as target cells, no medicines group was considered as negative control, Fluorouracil, Banzhen-1, Banzhen-1 + Fluorouracil, Banzhen-1 + Fluorouracil + TMP were considered as experiment groups. The expression of SGC-7901/ADR cells' MDR1 mRNA under different medicine groups was detected by the semi-quantitative RT- PC. Results The expression of MDR1 mRNA of Fluorouracil was the same as negative control (P > 0.05), however, the expression of MDR1 mRNA in other groups were different from each other and the group of Banzhen-1 + Fluorouracil + TMP was lowest (P﹤0.05). Conclusion It is possible correlated to MDR1 for Ban Zhen 1, TMP and Fluorouracil reversed multidrug resistance in human gastric cancer cells, which may supply a new theoretical basis for the clinical effective treatment of gastric carcinoma.
[Key words] Stomach cancer; Ban Zhen 1; Tetramethylpyrazine (TMP); Fluorouracil; Multidrug resistance gene 1 (MDR1)
化疗是治疗胃癌的有效手段之一，但胃癌多药耐药性(MDR)是导致化疗失败的主要原因。近几年发现MDR1在胃癌多药耐药性方面起着重要作用，侯培珍等[1]已证实斑贞1号 + 川芎嗪(TMP) + 氟尿嘧啶对人胃癌SGC-7901/ADR细胞有较强的逆转作用。因此本文笔者在此实验的基础上，通过检测不同药物组MDR1 mRNA的表达量从分子生物的角的进一步探讨斑贞1号 + TMP + 氟尿嘧啶对人胃癌SGC-7901/ADR细胞的作用机制 ......
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