HPLC测定伸筋壮骨胶囊中士的宁的含量(1)
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[摘要] 目的 用HPLC法建立伸筋壮骨胶囊中士的宁的含量测定方法。 方法 色谱柱为Diamonsil C18(250 mm × 4.6 mm，5 μm)；流动相为乙腈—0.01 mol/L庚烷磺酸钠与0.02 mol/L磷酸二氢钾的等量混合液(用10%磷酸调节pH=2.8)(21∶79)，流速1.0 mL/min，柱温35℃，检测波长为254 nm。 结果 士的宁在0.160 4～3.028 0 μg范围内线性良好，平均加样回收率为99.06%、RSD = 0.46%。 结论 本方法简便、准确、重现性好，适用于该制剂的质量控制。
[关键词] 伸筋壮骨胶囊；士的宁；含量测定；HPLC
[中图分类号] R927.2 [文献标识码] A [文章编号] 1674—4721(2012)09(c)—0053—02
Determination of strychnine in Shenjinzhuanggu Capsule by HPLC
CHEN Hao LIU Hang
The Food and Drug Inspection Office of Leshan City in Sichuan Province， Leshan 614000， China
[Abstract] Objective To establish a determination method of strychnine in Shenjinzhuanggu Capsule by HPLC. Methods The determination was carried out with Diamonsil C18 column (250 × 4.6 mm， 5 mm)； the mobile phase was acetonitrile—the mixed liquor composed of 0.01 mol/L heptane sulfonate as well as 0.02 mol/L potassium dihydrogen phosphate in equal measure (PH value of 2.8 was adjusted with 10% phosphoric acid) (21∶79) at a flow velocity of 1.0 mL/min， the column temperature was 35℃， and the detection wavelength was 254 nm. Results The amount of strychnine showed a good linear relationship between 0.160 4—3.028 0 μg. The average recoveries was 99.06% (RSD = 0.46%). Conclusion The method is convenient， sensitive， precise and well—repeatable， which can be appropriately used for quality control of Shenjinzhuanggu Capsule.
[Key words] Shenjinzhuanggu Capsule； Strychnine； Determination； High performance liquid chromatography
伸筋壮骨胶囊为峨眉山市中医院医院制剂，全方由马钱子、骨碎补、地龙等10味中药组成，具有舒筋壮骨、散结止痛的功效，可用于骨折筋伤后期，寒湿滞留经络所致的软组织硬化、肿胀，酸痛无力或风湿痛、神经痛等。方中马钱子为要药且为毒性中药材[1—5]。本文采用HPLC法测定伸筋壮骨胶囊中士的宁的含量，方法灵敏、专属性强、结果准确可靠，可用于伸筋壮骨胶囊中马钱子的质量控制。现报道如下：
1 仪器与试药
1.1 仪器
CARY100型紫外分光光度计；Agilent 1100 高效液相色谱仪 ......
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