人胆囊癌裸鼠移植瘤模型的建立及超声观察(1)
[摘要] 目的 建立人胆囊癌裸鼠皮下移植瘤模型，并对其进行超声观察。 方法 培养GBC-SD细胞，将细胞浓度调至1×107/ml，取0.2 ml注射于裸鼠皮下。成瘤后作为瘤源，瘤块剪成约2 mm3的组织块，置入裸鼠皮下，待其成瘤。以后各代模型的瘤源均来自上一代模型，用同样的方法接种。记录瘤块成瘤率、成瘤时间、生长特点。观察瘤块外观，并进行超声、解剖及病理观察。 结果 细胞注射法及组织块法可成功建立胆囊癌裸鼠移植瘤模型，成瘤率为100%；各代组织块法之间成瘤时间差异无统计学意义(P>0.05)；二维超声测量瘤体最大径与大体游标卡尺测量差异有统计学意义(P<0.05)。 结论 将细胞注射法与组织块法结合，是一种理想的建立人胆囊癌裸鼠移植瘤模型的实验方法。
[关键词] 胆囊癌；裸鼠；移植瘤；动物模型
[中图分类号] R735.8 [文献标识码] A [文章编号] 1674-4721(2016)02(b)-0004-04
Establishment of human gallbladder cancer nude-mouse transplanted tumor model and its ultrasonic observation
CHEN Juan ZHANG Yan-jing MIAO Yan ZHAO Yu-fang LIU Li-ping▲
Department of Ultrasound，the First Hospital of Shanxi Medical University，Taiyuan 030000，China
[Abstract] Objective To establish the human gallbladder cancer nude-mouse transplanted tumor model and to observe by ultrasound. Methods GBC-SD cells were cultured and the cell concentration was adjusted to 1×107/ml，of which 0.2 ml was subcutaneously injected to nude mouse.The cultivated tumor was selected as the tumor source and cut into tissue blocks of 2 mm3，which were then implanted into the subcutaneous tissue of nude mouse to cultivate tumor.The tumors of later generation were all cultivated from sources of tumors of the former generation by the same method.The tumor formation rate，time of tumor formation，and features of tumor formation were recorded.The appearance of tumor block was observed and ultrasonic，anatomical and pathological observations were conducted. Results The method of cell injection and tissue block implantation could successfully establish the gallbladder cancer nude-mouse transplanted tumor model，with tumor formation rate of 100%.The tumor formation time of each generation showed no statistically significant difference (P>0.05).The maximum diameter of tumor detected by ultrasound and vernier caliper showed statistically significant differences (P<0.05). Conclusion Combination of cell injection and tissue block implantation is a favorable method to establish the gallbladder cancer nude-mouse transplanted tumor model.
[Key words] Gallbladder cancer；Nude mouse；Transplanted tumor；Animal model
胆囊癌是胆道系统恶性肿瘤中最常见的一种类型，占消化道恶性肿瘤的第5位[1]。近年来，胆囊癌的发病率有逐渐增高的趋势，且早期原发性胆囊癌无特异性的临床表现，临床发现的胆囊癌多为中晚期，根治性切除率低，治疗效果及预后差[2-4]。因此建立合适的动物模型是研究胆囊癌体内生长、转移机制的重要工具，对胆囊癌的早期诊断、治疗及有效评估治疗效果、预后等有深远意义。本研究将GBC-SD细胞注射法和瘤组织块法结合，成功建立了裸鼠移植瘤模型，现报道如下。
1 材料与方法
1.1 细胞培养
GBC-SD人胆囊癌细胞购自中国科学院细胞库，细胞株从一位61岁的男性低分化胆囊癌患者中建立。细胞培养于含10%胎牛血清(杭州四季青公司)，0.4%双抗的RPMI1640(Gibco公司)培养基中，置于培养箱中连续培养及传代，培养箱温度保持在37℃，培养环境为5.0%CO2，1～2 d换液1次，细胞处于对数生长期时用胰酶消化后收集，加入适量生理盐水调整细胞悬液浓度至1×107/ml，置于1 ml的EP管中备用。, 百拇医药(陈娟 张炎晶 苗燕 赵育芳 刘利平)
[关键词] 胆囊癌；裸鼠；移植瘤；动物模型
[中图分类号] R735.8 [文献标识码] A [文章编号] 1674-4721(2016)02(b)-0004-04
Establishment of human gallbladder cancer nude-mouse transplanted tumor model and its ultrasonic observation
CHEN Juan ZHANG Yan-jing MIAO Yan ZHAO Yu-fang LIU Li-ping▲
Department of Ultrasound，the First Hospital of Shanxi Medical University，Taiyuan 030000，China
[Abstract] Objective To establish the human gallbladder cancer nude-mouse transplanted tumor model and to observe by ultrasound. Methods GBC-SD cells were cultured and the cell concentration was adjusted to 1×107/ml，of which 0.2 ml was subcutaneously injected to nude mouse.The cultivated tumor was selected as the tumor source and cut into tissue blocks of 2 mm3，which were then implanted into the subcutaneous tissue of nude mouse to cultivate tumor.The tumors of later generation were all cultivated from sources of tumors of the former generation by the same method.The tumor formation rate，time of tumor formation，and features of tumor formation were recorded.The appearance of tumor block was observed and ultrasonic，anatomical and pathological observations were conducted. Results The method of cell injection and tissue block implantation could successfully establish the gallbladder cancer nude-mouse transplanted tumor model，with tumor formation rate of 100%.The tumor formation time of each generation showed no statistically significant difference (P>0.05).The maximum diameter of tumor detected by ultrasound and vernier caliper showed statistically significant differences (P<0.05). Conclusion Combination of cell injection and tissue block implantation is a favorable method to establish the gallbladder cancer nude-mouse transplanted tumor model.
[Key words] Gallbladder cancer；Nude mouse；Transplanted tumor；Animal model
胆囊癌是胆道系统恶性肿瘤中最常见的一种类型，占消化道恶性肿瘤的第5位[1]。近年来，胆囊癌的发病率有逐渐增高的趋势，且早期原发性胆囊癌无特异性的临床表现，临床发现的胆囊癌多为中晚期，根治性切除率低，治疗效果及预后差[2-4]。因此建立合适的动物模型是研究胆囊癌体内生长、转移机制的重要工具，对胆囊癌的早期诊断、治疗及有效评估治疗效果、预后等有深远意义。本研究将GBC-SD细胞注射法和瘤组织块法结合，成功建立了裸鼠移植瘤模型，现报道如下。
1 材料与方法
1.1 细胞培养
GBC-SD人胆囊癌细胞购自中国科学院细胞库，细胞株从一位61岁的男性低分化胆囊癌患者中建立。细胞培养于含10%胎牛血清(杭州四季青公司)，0.4%双抗的RPMI1640(Gibco公司)培养基中，置于培养箱中连续培养及传代，培养箱温度保持在37℃，培养环境为5.0%CO2，1～2 d换液1次，细胞处于对数生长期时用胰酶消化后收集，加入适量生理盐水调整细胞悬液浓度至1×107/ml，置于1 ml的EP管中备用。, 百拇医药(陈娟 张炎晶 苗燕 赵育芳 刘利平)