尿沉渣分析仪SysmexUF-500i在快速筛查尿路感染中的应用价值(1)
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【摘要】 目的:以中段尿定量细菌培养为金标准,评价Sysmex UF-500i尿沉渣分析仪(简称UF-500i)细菌定量计数参数在快速筛查尿路感染(Urinary tract infection,UTI)中的应用价值,为临床经验用药提供参考。方法:采集260份中段尿标本,以尿液细菌培养菌落计数阳性为诊断UTI金标准,比较分析UF-500i细菌定量计数结果,并计算其诊断UTI的敏感性、特异性、阴性及阳性预测值、假阳性及假阴性率和诊断准确率。结果:260份尿液标本中细菌培养阳性87例,占33.4%;以UF-500i细菌定量计数临界值3750/μl为筛查UTI的诊断临界值,UF-500i细菌定量计数与定量培养结果比较差异无统计学意义(P>0.05),其诊断UTI的敏感性为85.1%,特异性为91.3%,阳性预计值为87.0%,阴性预计值为92.5%,假阳性率为8.7%,假阴性率为14.9%,准确率为90.7%。在尿培养阳性的前两位致病菌中,两种方法定量计数大肠埃希菌和克雷伯菌属细菌的相关性分别为0.990和0.989。结论:UF-500i操作简便、快速和准确度高,在一定范围内可作为UTI的快速筛检指标和指导临床经验用药。
【关键词】 尿液流式细胞分析仪; 细菌培养; 尿路感染
Application of UF-500i Urine Sedimentation Analyze in Screening Urinary Tract Infection/CHEN Hong-bing,ZHU Cheng-bin,SUN Wei.//Medical Innovation of China,2012,9(33):089-090
【Abstract】 Objective:To compare the UF-500i urine sedimentation analyze (UF-500i) with urine bacterial culture in screening urinary tract infection (UTI),and to provide a reference for the clinical experience of drug use.Method:260 fresh midstream urine were collected and examined by cultures and counts of bacterial colonies and UF-500i analysis.The results between the two methods were compared,and then the sensitivity,specificity,negative/positive predictive value,false positive/negative rate and accuracy were calculated.Result:The positive rate of urine culture was 87 patients (33.4%) and there was no significant difference between UF-500i bacterial quantitative counts and urine bacterial cultures (P>0.05) when the bacterial quantitative counts of 3750/μL was used to screen UTI diagnosis threshold.The sensitivity of UF-500i was 85 ......
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