五味子酚上调SIRT1改善H2O2对SH—SY5Y细胞增殖的抑制作用研究(1)
【摘要】 目的：探討SIRT1/LC3自噬通路在五味子酚(Schisanhenol，Sal)抗H2O2诱导的SH-SY5Y细胞增殖活力下降中的作用。方法：加入不同浓度Sal(50、10 μmol/L)处理SH-SY5Y细胞4 h后，再加入H2O2 100 μmol/L处理12 h，采用MTT法检测细胞增殖能力，Western blot检测SIRT1蛋白和自噬蛋白标志物(LC3蛋白、Beclin-1蛋白)的表达水平。结果：H2O2组细胞增殖活力较对照组显著下降(P<0.001)；Sal组不同浓度(50、10 μmol/L)干预后的细胞增殖活力均高于H2O2组(P=0.001、0.017)，且随着Sal浓度增加，细胞增殖活力增强。H2O2组SIRT1蛋白含量较对照组明显降低(P<0.001)，Sal组不同浓度(50、10 μmol/L)的SIRT1蛋白含量均明显高于H2O2组(P=0.001、0.014)。H2O2组Beclin-1和LC3-Ⅱ蛋白表达均高于对照组，比较差异均有统计学意义(P<0.001)；Sal组(50 μmol/L)的Beclin-1的蛋白表达低于H2O2组，且不同浓度(50、10 μmol/L)LC3-Ⅱ蛋白表达均低于H2O2组，比较差异均有统计学意义(P<0.05)。结论：Sal可通过上调SIRT1蛋白表达，拮抗H2O2引起的过度自噬，从而起到拮抗H2O2对SH-SY5Y细胞增殖的抑制作用。
, 百拇医药
【关键词】 五味子酚； SIRT1； 过氧化氢； LC3
Study on the Inhibitory Effect of Schisanhenol Up-regulated SIRT1 Improve H2O2 on the Proliferation of SH-SY5Y Cells/YANG Hongyan，MA Shuli，LI Qingyi，et al.//Medical Innovation of China，2018，15(06)：032-035
【Abstract】 Objective：To investigate the role of SIRT1/LC3 autophagy pathway in the decrease of proliferation activity of SH-SY5Y cells induced by H2O2 in Schisanhenol(Sal).Method：SH-SY5Y cells were treated with different concentrations of Sal(50 and 10 μmol/L) for 4 h and then added H2O2 100 μmol/L for 12 h.
, 百拇医药
The cell proliferation activity was detected by MTT，and the expressions of SIRT1 protein and autophagic protein markers(LC3 protein，Beclin-1 protein) were detected by Western blot.Result：The cell proliferation activity of H2O2 group was significantly lower than that of control group(P<0.001)，the proliferative activity of Sal group at different concentrations(50，10 μmol/L) was higher than that of H2O2 group(P=0.001，0.017)，and the cell proliferation activity increased with the increase of Sal concentration.The content of SIRT1 protein in H2O2 group was significantly lower than that of control group(P<0.001)，and the content of SIRT1 protein in Sal group at different concentrations(50，10 μmol/L) was significantly higher than that of H2O2 group(P=0.001，0.014).The expressions of Beclin-1 and LC3-Ⅱ proteins in group H2O2 were higher than those of control group，the differences were statistically significant(P<0.001)，the protein expression of Beclin-1 in Sal group(50 μmol/L) was lower than that of H2O2 group，and the expression of LC3-Ⅱ protein in different concentrations(50，10 μmol/L) was lower than that in H2O2 group，the differences were statistically significant(P<0.05).Conclusion：Sal can attenuate the excessive autophagy induced by H2O2 by up regulation of the expression of SIRT1 protein，thus antagonizing the inhibitory effect of H2O2 on the proliferation of SH-SY5Y cells., 百拇医药(杨宏艳 马淑丽 李清漪 都晓辉 韩云峰 孙辑凯 包亚男)
, 百拇医药
【关键词】 五味子酚； SIRT1； 过氧化氢； LC3
Study on the Inhibitory Effect of Schisanhenol Up-regulated SIRT1 Improve H2O2 on the Proliferation of SH-SY5Y Cells/YANG Hongyan，MA Shuli，LI Qingyi，et al.//Medical Innovation of China，2018，15(06)：032-035
【Abstract】 Objective：To investigate the role of SIRT1/LC3 autophagy pathway in the decrease of proliferation activity of SH-SY5Y cells induced by H2O2 in Schisanhenol(Sal).Method：SH-SY5Y cells were treated with different concentrations of Sal(50 and 10 μmol/L) for 4 h and then added H2O2 100 μmol/L for 12 h.
, 百拇医药
The cell proliferation activity was detected by MTT，and the expressions of SIRT1 protein and autophagic protein markers(LC3 protein，Beclin-1 protein) were detected by Western blot.Result：The cell proliferation activity of H2O2 group was significantly lower than that of control group(P<0.001)，the proliferative activity of Sal group at different concentrations(50，10 μmol/L) was higher than that of H2O2 group(P=0.001，0.017)，and the cell proliferation activity increased with the increase of Sal concentration.The content of SIRT1 protein in H2O2 group was significantly lower than that of control group(P<0.001)，and the content of SIRT1 protein in Sal group at different concentrations(50，10 μmol/L) was significantly higher than that of H2O2 group(P=0.001，0.014).The expressions of Beclin-1 and LC3-Ⅱ proteins in group H2O2 were higher than those of control group，the differences were statistically significant(P<0.001)，the protein expression of Beclin-1 in Sal group(50 μmol/L) was lower than that of H2O2 group，and the expression of LC3-Ⅱ protein in different concentrations(50，10 μmol/L) was lower than that in H2O2 group，the differences were statistically significant(P<0.05).Conclusion：Sal can attenuate the excessive autophagy induced by H2O2 by up regulation of the expression of SIRT1 protein，thus antagonizing the inhibitory effect of H2O2 on the proliferation of SH-SY5Y cells., 百拇医药(杨宏艳 马淑丽 李清漪 都晓辉 韩云峰 孙辑凯 包亚男)