维生素K3对尿路结石病人尿中骨桥蛋白浓度的影响(1)
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【摘要】 目的：探讨维生素K3对尿路结石病人尿中骨桥蛋白浓度的影响；从而观察维生素K3对尿路结石病人的治疗和预防复发的效果。方法：随机选取30例尿路结石患为维生素K组，肌注维生素K312mg/d，连续7天；收集用药前后的尿标本；随机选30例正常人做对照组，收集尿标本；利用western blotting技术测定各尿标本中的骨桥蛋白含量；比较尿路结石患者用药前后及与正常人尿中骨桥蛋白含量的差别。统计学方法：配对资料均数t检验。结果：维生素K组用药后比用药前尿中骨桥蛋白含量高18.7%(P<0.05)；用药前组比正常对照组尿中骨桥蛋白含量高93%，但差别无统计学意义(P=0.036)；用药后组比正常对照组尿中骨桥蛋白含量明显升高129%(P=0.0045，<0.01)。结论：临床应用维生素K可上调肾骨桥蛋白的表达，抑制结石的形成，对尿路结石的治疗和预防有一定效果，值得推广。
【关键词】 维生素K；雌二醇；尿路结石；骨桥蛋白；肾
Effect of Vitamin K3 on the concentration of osteopontin in the urine of the patients suffered from urolithiasis
Li min, Zhao Guofang, Liu meiyun
Department of Urology, Donghua Affiliated hospital, Sun Yat-sen University, Dongguan,Guangdong,523013
【Abstract】 Objective To investigate the effect of Vitamin K on concentration of osteopontin in the urine of patients suffered from urolithiasis; To investigate the effect of vitamin K in treating and preventing urolithiasis. Methods 30 patients who were suffered from urolithiasis were treated with vitamin K3 injection(12mg/d,for 7days) ; 30 normal persons for comparing; all urine specimens were collected in patients before and after treatment and in normal persons, respectively. The concentration of osteopontin in urine were detected with Western blotting. Statistics analysis is t test. Results The concentration of osteopontin in urine were 18.7% higher after vitamin K3 treated(P<0.05). The concentration of osteopontin in urine in group vitamin K before vitamin K used were 93% higher than normal group, but there was no significance in statistics(P=0.36). The concentration of osteopontin in urine in group vitamin K after vitamin K used were 129% higher than normal group,(P=0.045,P<0.05).Conclusion Vtamin K can clinically up-regulate the expression of OPN (osteopontin), and inhabit the formation of urolithiasis. Vtamin K have exact effect in treating and preventing urolithiasis, and could be extensively recommended.
【Key words】 Vitamin K, urolithiasis, kidney, OPN(osteopontin)
前言
泌尿系结石是一种常见疾病，治愈后有明显的复发倾向。红外光谱分析表明，86%的结石中含有草酸钙，其治疗后复发率高达60%～80%。因此，如何预防草酸钙结石的复发是个重要的临床课题。近年来的研究发现，给服用诱石剂的大鼠每天皮下注射维生素K34mg能减少肾草酸钙结晶的数量[1]。动物实验研究表明维生素K可通过上调尿中骨桥蛋白的表达来达到抑制结石形成的作用 ......
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