【摘要】目的：明确舰船火灾中有毒烟雾吸入后是否对肺组织有长期的损伤，并探讨其机制。方法：大鼠肺组织病理学HE染色检查，气管粘膜原代细胞培养及CK19免疫荧光染色，激光共聚焦显微镜观察。结果：与对照组相比，烟雾吸入后3d可引发急性肺损伤，烟雾吸入后3m可引发慢性肺损伤，烟雾吸入后3m组细胞胞质中CK19表达的绿色荧光明显增强。结论：有毒烟雾吸入后可对大鼠肺组织造成长期慢性损伤，并有可能导致支气管粘膜上皮细胞向恶性转化。

    【关键词】烟雾吸入；肺损伤；CK19

    Long term injury of lung tissue by smoke inhalation in rats after ship burning

    Ying Xie， Xue Chen， You Hu， Ying Wang*

    Huanghe North Avenue， Shenyang， Liaoning， Shenyang 110034， Liaoning， China

    Abstract： Objective： This study was to determine whether long-term damage to lung tissue is caused by inhalation of toxic fumes in ship fires and to explore mechanisms. Methods： Histopathological examination of lung tissue in rats was performed by HE ......