[摘要] [目的] 从棒曲霉菌培养上清液中分离纯化具有抗真菌活性的肽类物质(AFP). [方法] 用离子交换柱层析法分离AFP，利用反相高效液相色谱法进一步纯化，经聚丙烯酰胺凝胶电泳分析确定相对分子质量. [结果] 从棒曲霉菌培养上清液中分离出1种具有抗真菌活性的AFP，纯化后得到高活性的精制AFP，相对分子质量约为8ku，MTT法测定结果表明AFP对5种真菌均具有明显的抗真菌作用. [结论] 棒曲霉菌培养上清液中含具有抗真菌活性的AFP.

    [关键词] 棒曲霉菌;分离;真菌蛋白类

    CAI Ying-ji 1 ，JIN Zhe-zhu 2*

    (1.Health Science Center;2.Editorial Board of Journal of Medical Science:Yanbian University，Yanji133000，Jilin，China) ABSTRACT:OBJECTIVE To purify the peptide with antifungal activity from Aspergillus clavalus desm culture fluid.METHODS The peptide was separated by ion exchange column chromatography and further purified by reverse phase HPLC ......