不同种源地广金钱草药材质量变异与遗传多样性分析(4)
2. Kangmei Institute of Materia Medica， kangmei Pharmaceutical Co.， Ltd.， Guangzhou 510006， China)
[Abstract] Objective： To explore the quality variation and genetic diversity of Desmodium styracifolium from different provenances， and lay a foundation for rational exploitation on germplasm resources and fine variety breeding of D. styracifolium. Method： Amplified fragment length polymorphism (AFLP) markers were developed to analyze genetic diversity in D. styracifolium from 18 resources. NTSYSpc2.11F software was used to analyze the similarity among the D. styracifolium germplasms and construct the genetic phylogenetic tree. The schaftoside content in D. styracifolium from different provenances was determined by HPLC. Result： A total of 844 fragments were amplified with 8 primers， in which 717 were polymorphic bands， accounting for 84.27% of the total detected variation. All the specimens from 18 resources could be grouped into 3 clusters by cluster analysis. The schaftoside contents of D. styracifolium germplasms differed significantly， with the highest content in the germplasm from Sanya， Hainan. Conclusion： Significant quality variation and genetic diversity can be observed among D. styracifolium germplasms. The diverse germplasm resources should be explored and the fine variety should be selected to breed.
[Key words] Desmodium styracifolium； quality variance； genetic diversity
doi：10.4268/cjcmm20130917
[责任编辑 吕冬梅], 百拇医药(杨全，卢挺，桑雪雨，陈泉，张春荣，唐晓敏，程轩轩，李书渊)
[Abstract] Objective： To explore the quality variation and genetic diversity of Desmodium styracifolium from different provenances， and lay a foundation for rational exploitation on germplasm resources and fine variety breeding of D. styracifolium. Method： Amplified fragment length polymorphism (AFLP) markers were developed to analyze genetic diversity in D. styracifolium from 18 resources. NTSYSpc2.11F software was used to analyze the similarity among the D. styracifolium germplasms and construct the genetic phylogenetic tree. The schaftoside content in D. styracifolium from different provenances was determined by HPLC. Result： A total of 844 fragments were amplified with 8 primers， in which 717 were polymorphic bands， accounting for 84.27% of the total detected variation. All the specimens from 18 resources could be grouped into 3 clusters by cluster analysis. The schaftoside contents of D. styracifolium germplasms differed significantly， with the highest content in the germplasm from Sanya， Hainan. Conclusion： Significant quality variation and genetic diversity can be observed among D. styracifolium germplasms. The diverse germplasm resources should be explored and the fine variety should be selected to breed.
[Key words] Desmodium styracifolium； quality variance； genetic diversity
doi：10.4268/cjcmm20130917
[责任编辑 吕冬梅], 百拇医药(杨全，卢挺，桑雪雨，陈泉，张春荣，唐晓敏，程轩轩，李书渊)