白芍水提部位化学成分的HPLC—ESI—Q—TOF—MS分析(5)
4 讨论
本实验的色谱分离结果显示色谱峰没有完全分开。课题组采用LC-MS/MS联用的技术，利用质谱的高分辨率优势，精确质量测定的定性能力等优点，通过获得色谱峰的准分子离子峰，对白芍水提取部分进行了化学成分定性分析，找出白芍水提取部分可能含有的25种化学成分。其成分再通过二级质谱，并依据特征碎片离子峰加以确认。在正负离子模式检测到的化学成分中，根据准确相对分子质量(-101*
(1.College of Pharmacy， Nanjing University of Chinese Medicine， Nanjing 210046， China；
2.School of Pharmacy， Shaanxi University of Chinese Medicine， Xianyang 712046， China)
[Abstract] This experiment was performed to establish a qualitative analysis on chemical composition in water extract of Paeoniae Radix Alba by HPLC-ESI-Q-TOF-MS. The analysis was conducted on a C18 (Hanbon LichrospherTM， 4.6 mm×250 mm， 5 μm) column with methanol-0.1% formic acid as the mobile phase for gradient elution； ESI ion source was used for mass spectra， and data were collected in both positive and negative modes. The results showed that eleven compounds from water extract of Paeoniae Radix Alba had been identified by analyzing positive and negative ion mass data including element composition and by comparing with data from literatures. Since efficient separation of HPLC and the high sensitive detection of MS was used， this experiment， it will provide evidences for elucidation of the effective substance in the water extract of Paeoniae Radix Alba.
[Key words] Paeoniae Radix Alba； chemical components； HPLC-ESI-Q-TOF-MS
doi：10.4268/cjcmm20131123
[责任编辑 孔晶晶], 百拇医药(孙东东,徐晓芳,崔九成,宋小妹,李祥)
本实验的色谱分离结果显示色谱峰没有完全分开。课题组采用LC-MS/MS联用的技术，利用质谱的高分辨率优势，精确质量测定的定性能力等优点，通过获得色谱峰的准分子离子峰，对白芍水提取部分进行了化学成分定性分析，找出白芍水提取部分可能含有的25种化学成分。其成分再通过二级质谱，并依据特征碎片离子峰加以确认。在正负离子模式检测到的化学成分中，根据准确相对分子质量(-101*
(1.College of Pharmacy， Nanjing University of Chinese Medicine， Nanjing 210046， China；
2.School of Pharmacy， Shaanxi University of Chinese Medicine， Xianyang 712046， China)
[Abstract] This experiment was performed to establish a qualitative analysis on chemical composition in water extract of Paeoniae Radix Alba by HPLC-ESI-Q-TOF-MS. The analysis was conducted on a C18 (Hanbon LichrospherTM， 4.6 mm×250 mm， 5 μm) column with methanol-0.1% formic acid as the mobile phase for gradient elution； ESI ion source was used for mass spectra， and data were collected in both positive and negative modes. The results showed that eleven compounds from water extract of Paeoniae Radix Alba had been identified by analyzing positive and negative ion mass data including element composition and by comparing with data from literatures. Since efficient separation of HPLC and the high sensitive detection of MS was used， this experiment， it will provide evidences for elucidation of the effective substance in the water extract of Paeoniae Radix Alba.
[Key words] Paeoniae Radix Alba； chemical components； HPLC-ESI-Q-TOF-MS
doi：10.4268/cjcmm20131123
[责任编辑 孔晶晶], 百拇医药(孙东东,徐晓芳,崔九成,宋小妹,李祥)