丹参酮ⅡA磺酸钠对AngⅡ诱导心房成纤维细胞胶原合成及TGF—β1活化的影响(4)
2. Department of Anesthesiology， Union Hospital of Huazhong University of Science and Technology， Wuhan 430022， China；
3. Department of Pharmacology， Tongji Medical College of Huazhong University of
Science and Technology， Wuhan 430030， China)
[Abstract] Objective： To observe the effect of sodium tanshinone ⅡA sulfonate (STS) on Ang Ⅱ-induced atrial fibroblast collagen synthesis and TGF-β1 activation. Method： Atrial fibroblasts of neonatal rats were cultured to determine the content of collagen protein. The original synthesis rate determined by the [3H]-proline incorporation method was taken as the index for myocardial fibrosis. The content of active TGF-β1 and total TGF-β1 in cell culture supernatants were tested and cultured by ELISA. The expression of thrombospondin-1 (TSP-1) was assessed by using Western blot. Result： Ang Ⅱ could significantly increase the content of atrial fibroblast collagen and the collagen synthesis rate， the TSP-1 expression and the concentration of active TGF-β1， without any obvious change in total TGF-β1. After the STS treatment， all of the indexes， apart from total TGF-β1， were obviously down-regulated. Conclusion： STS could decrease the secretion of Ang Ⅱ-induced atrial fibroblast collagen and the synthesis rate. Its mechanism is related to the inhibition of TSP-1/TGF-β1 pathway.
[Key words] sodium tanshinoneⅡA sulfonate； atrial fibrosis； transforming growth factor-β1
doi：10.4268/cjcmm20140627, 百拇医药(杨乐 邹晓静 尹钊 郝洪真)
3. Department of Pharmacology， Tongji Medical College of Huazhong University of
Science and Technology， Wuhan 430030， China)
[Abstract] Objective： To observe the effect of sodium tanshinone ⅡA sulfonate (STS) on Ang Ⅱ-induced atrial fibroblast collagen synthesis and TGF-β1 activation. Method： Atrial fibroblasts of neonatal rats were cultured to determine the content of collagen protein. The original synthesis rate determined by the [3H]-proline incorporation method was taken as the index for myocardial fibrosis. The content of active TGF-β1 and total TGF-β1 in cell culture supernatants were tested and cultured by ELISA. The expression of thrombospondin-1 (TSP-1) was assessed by using Western blot. Result： Ang Ⅱ could significantly increase the content of atrial fibroblast collagen and the collagen synthesis rate， the TSP-1 expression and the concentration of active TGF-β1， without any obvious change in total TGF-β1. After the STS treatment， all of the indexes， apart from total TGF-β1， were obviously down-regulated. Conclusion： STS could decrease the secretion of Ang Ⅱ-induced atrial fibroblast collagen and the synthesis rate. Its mechanism is related to the inhibition of TSP-1/TGF-β1 pathway.
[Key words] sodium tanshinoneⅡA sulfonate； atrial fibrosis； transforming growth factor-β1
doi：10.4268/cjcmm20140627, 百拇医药(杨乐 邹晓静 尹钊 郝洪真)