儿童急性淋巴细胞白血病患者细胞因子IL—2和IL—10水平变化的临床研究(1)
摘要：目的 探讨儿童急性淋巴细胞白血病(ALL)患者初步诊断时和化疗完全缓解(CR)后血清中IL-2、IL-10水平的变化。方法 收集确诊为ALL且经过化疗获得完全缓解的患儿30例及健康儿童20例，采用流式细胞术(FCM)测量初诊组、完全缓解组(CR)、健康对照组血清中IL-2、IL-10的水平，比较化疗前后这两种细胞因子含量的变化，并分别与健康对照组做对比。结果 初诊组患儿IL-2水平( 3.59±1.18)pg/ml与正常对照组儿童IL-2水平(6.22±2.03)pg/ml相比较明显下降( P<0.001)，初诊组IL-10的水平(15.88±4.73)pg/ml与正常儿童IL-10的水平( 5.69±3.44 )pg/ml相比较则显著升高(P<0.001)。随着化疗的进行IL-2水平( 4.78±1.57 )pg/ml明显上升，(P=0.005)，IL-10水平( 8.14±2.82 )pg/ml显著下降( P<0.001)，都未达到正常儿童水平(P= 0.034和P=0.036)。但是差异均有统计学意义(P<0.05)。结论 细胞因子IL-2和IL-10可能在儿童ALL的发生、发展过程中发挥了免疫调节功能，其含量的变化在一定程度上能够反应儿童ALL患儿体内的免疫功能状态的好坏。
, 百拇医药
关键词：儿童；急性淋巴细胞白血病；IL-2IL-10；流式细胞术
Abstract：Objective To investigate the children with acute lymphoblastic leukemia(ALL)patients at initial diagnosis and chemotherapy complete remission(CR)after IL-2 in serum，the change of IL-10 level.Methods Diagnosed with ALL after chemotherapy and 20 cases of complete remission in 30 cases of children and healthy children by flow cytometry(FCM)measurement diagnosis group，complete remission group(CR)，healthy control group serum IL-2，IL-10 level changes of these two cytokines were compared before and after chemotherapy，and compared with the healthy control group respectively.Results The level of IL-2(3.59±1.18) pg/ml in the newly diagnosed group was significantly lower than that in the normal control group (6.22±2.03) pg/ml(P<0.001).The level of IL-10 in the first visit group(15.88±4.73)pg/ml was significantly higher than that of the normal children (5.69±3.44)，IL-10(P<0.001)，compared with the normal children.With chemotherapy were IL-2 level (4.78±1.57)pg/ml significantly increased(P=0.005)，IL-10(8.14±2.82)pg/ml decreased significantly(P<0.001)，has not reached the level of normal children(P= 0.034 and P=0.036).But the differences were statistically significant(P<0.05).Conclusion The cytokines IL-2 and IL-10 may play an immunoregulatory function in the development and progression of ALL in children.The changes of their contents can reflect the immune function in children with ALL.
, 百拇医药
Key words：Children；Acute lymphoblastic leukemia；IL-2IL-10；Flow cytometry
近年來，有许多关于白血病患者体内细胞因子变化的研究，但大多数实验的对象以成人为主，也有部分实验的对象包括成人和儿童，并且儿童的平均年龄偏大，很少完全以儿童为主要对象的实验研究。儿童急性白血病尤其是低年龄段的患者数量呈逐年增加趋势，且儿童免疫功能尚未完善，因此，了解儿童免疫功能状态十分必要。儿童急性淋巴细胞白血病(ALL)是儿童恶性肿瘤中最常见的疾病，以骨髓中白血病细胞克隆性增生为主，抑制了正常造血细胞的产生，并侵犯其他组织和器官，从而使患儿表现出贫血、出血、感染、骨痛、关节浸润等一系列临床症状，且起病急，病情进展快，病程短，易复发，给家庭带来了巨大的经济负担。目前白血病的发病机理尚不十分明确，可能与染色体异常、化工燃料、病毒感染、辐射、遗传易感性等因素相关。有研究[1]证实细胞因子的分泌和调节异常能使肿瘤细胞逃脱机体的免疫监视，细胞因子的网络失衡可能为急性白血病的发病提供机会[2]。现采用流式细胞术对我院30名ALL患儿化疗前及完全缓解后血清中细胞因子IL-2和IL-10的含量进行测定，探讨这两种细胞因子水平的变化与儿童ALL患者体内免疫功能状态的关系。, http://www.100md.com(唐会涛 郝国平)
, 百拇医药
关键词：儿童；急性淋巴细胞白血病；IL-2IL-10；流式细胞术
Abstract：Objective To investigate the children with acute lymphoblastic leukemia(ALL)patients at initial diagnosis and chemotherapy complete remission(CR)after IL-2 in serum，the change of IL-10 level.Methods Diagnosed with ALL after chemotherapy and 20 cases of complete remission in 30 cases of children and healthy children by flow cytometry(FCM)measurement diagnosis group，complete remission group(CR)，healthy control group serum IL-2，IL-10 level changes of these two cytokines were compared before and after chemotherapy，and compared with the healthy control group respectively.Results The level of IL-2(3.59±1.18) pg/ml in the newly diagnosed group was significantly lower than that in the normal control group (6.22±2.03) pg/ml(P<0.001).The level of IL-10 in the first visit group(15.88±4.73)pg/ml was significantly higher than that of the normal children (5.69±3.44)，IL-10(P<0.001)，compared with the normal children.With chemotherapy were IL-2 level (4.78±1.57)pg/ml significantly increased(P=0.005)，IL-10(8.14±2.82)pg/ml decreased significantly(P<0.001)，has not reached the level of normal children(P= 0.034 and P=0.036).But the differences were statistically significant(P<0.05).Conclusion The cytokines IL-2 and IL-10 may play an immunoregulatory function in the development and progression of ALL in children.The changes of their contents can reflect the immune function in children with ALL.
, 百拇医药
Key words：Children；Acute lymphoblastic leukemia；IL-2IL-10；Flow cytometry
近年來，有许多关于白血病患者体内细胞因子变化的研究，但大多数实验的对象以成人为主，也有部分实验的对象包括成人和儿童，并且儿童的平均年龄偏大，很少完全以儿童为主要对象的实验研究。儿童急性白血病尤其是低年龄段的患者数量呈逐年增加趋势，且儿童免疫功能尚未完善，因此，了解儿童免疫功能状态十分必要。儿童急性淋巴细胞白血病(ALL)是儿童恶性肿瘤中最常见的疾病，以骨髓中白血病细胞克隆性增生为主，抑制了正常造血细胞的产生，并侵犯其他组织和器官，从而使患儿表现出贫血、出血、感染、骨痛、关节浸润等一系列临床症状，且起病急，病情进展快，病程短，易复发，给家庭带来了巨大的经济负担。目前白血病的发病机理尚不十分明确，可能与染色体异常、化工燃料、病毒感染、辐射、遗传易感性等因素相关。有研究[1]证实细胞因子的分泌和调节异常能使肿瘤细胞逃脱机体的免疫监视，细胞因子的网络失衡可能为急性白血病的发病提供机会[2]。现采用流式细胞术对我院30名ALL患儿化疗前及完全缓解后血清中细胞因子IL-2和IL-10的含量进行测定，探讨这两种细胞因子水平的变化与儿童ALL患者体内免疫功能状态的关系。, http://www.100md.com(唐会涛 郝国平)
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