机用镍钛器械M3联合次氯酸钠抑制根管内生物膜的研究(1)
摘 要：目的 研究机用镍钛器械M3联合次氯酸钠抑制根管内生物膜的效果。方法 选取我院自2015年4月～2016年4月收集的80例患有慢性根尖周病的患者作为研究对象，随机将患者分为对照组和观察组，各40例，其中对照组采用次氯酸钠不锈钢锉进行冲洗，观察组患者则采用机用镍钛器械M3联合次氯酸钠进行冲洗，比较两组根管内生物膜的生长情况。结果 观察组患者根管内生物膜完好的有0例，生物膜完全消失的有22例(55.00%)；显著优于对照组的12例(30.00%)、8例(20.00%)；观察组患者在次氯酸钠冲洗后细菌存活率为5.00%(2例)，显著低于对照组的52.50%(21例)(P<0.05)。结论 机用镍钛器械M3联合次氯酸钠可更好额抑制根管内生物膜的生长，最大程度的发挥次氯酸钠的杀菌、消毒作用，破坏细菌生物膜，降低细菌生存率，可在临床上可在临床广泛推荐应用。
关键词：机用镍钛器械M3；次氯酸钠；根管内生物膜
中图分类号：R783.1 文献标识码：A DOI：10.3969/j.issn.1006-1959.2018.02.029
, http://www.100md.com
文章编号：1006-1959(2018)02-0086-03
Abstract：Objective To study the effect of machine nickel-titanium instrument M3 combined with sodium hypochlorite on inhibiting biofilm in root canal.Methods A total of 80 patients with chronic apical disease collected in our hospital from April 2015 to April 2016 were selected as study subjects and randomly divided into control group and observation group with 40 cases in each group.The control group was flushed with sodium hypochlorite stainless steel file，while the patients in the observation group were washed with Nickel-titanium instrument M3 combined with sodium hypochlorite.The biofilm growth in the two groups was compared.Results In the observation group， there were 0 cases with biofilm intact and 22 cases with complete disappearance of biofilm(55.00%)，12 cases(30.00%)were significantly better than the control group and 8 cases(20.00% ).The survival rate of bacteria in the observation group after the sodium hypochlorite flushing was 5.00% (2 cases)， which was significantly lower than that in the control group52.50%(21 cases)(P<0.05).Conclusion The nickel - titanium device M3 combined with sodium hypochlorite can better inhibit the growth of the biofilm in the root canal，exert the sterilization and disinfection functions of the sodium hypochlorite to the maximum extent，destroy the bacterial biofilm and reduce the bacterial survival rate，and can be clinically widely recommended.
, http://www.100md.com
Key words：Nickel titanium apparatus M3；Sodium hypochlorite；Endodontic biofilm
根尖周病變(periapical disease)是指发生在根管内，牙根周围组织，如牙本质、牙骨质、根尖周围的牙周膜和牙槽骨等组织之间的疾病，其主要致病因素为细菌感染[1]。研究表明，生物膜在根管内生长是慢性根尖周炎和根管再感染的主要病因，因此抑制根管内生物膜的形成尤为重要[2]。比对各种大量相关的临床资料表明，临床上常用的冲洗剂—次氯酸钠，不仅能够具有很强的杀菌效果，还能有效溶解腐败坏死组织，在一定程度上清除感染，但注射方式还有待考究[3-4]。常规不锈钢锉注射不均匀，杀菌效果还有待提高，随着医学的发展，各类医学仪器的出现，镍钛器械M3被引入到临床应用当中，目前效果反响良好。鉴于此，我院特做了此次研究，现将结果报告如下。
1资料与方法
1.1一般资料 选取我院自2015年4月～2016年4月收集的80例患有慢性根尖周病的患者作为研究对象，所有患者均经临床确诊为慢性根尖周炎，且经牙髓点活力检测为阴性。两组患者在近两年内牙齿均无外伤且无牙冠缺损严重和牙周疾病等现象，所有程序经过医院伦理委员会审核。随机将患者分为对照组和观察组两组，每组40例。其中对照组患者男23例，女17例，年龄18～63岁，平均年龄(38.34±3.21)岁；观察组患者男22例，女18例，年龄16～62岁，平均年龄(37.27±3.53)岁。两组患者一般资料对比，差异无统计学意义(P>0.05)，具有可比性。, http://www.100md.com(付爽 杨东红 宁尚波)
关键词：机用镍钛器械M3；次氯酸钠；根管内生物膜
中图分类号：R783.1 文献标识码：A DOI：10.3969/j.issn.1006-1959.2018.02.029
, http://www.100md.com
文章编号：1006-1959(2018)02-0086-03
Abstract：Objective To study the effect of machine nickel-titanium instrument M3 combined with sodium hypochlorite on inhibiting biofilm in root canal.Methods A total of 80 patients with chronic apical disease collected in our hospital from April 2015 to April 2016 were selected as study subjects and randomly divided into control group and observation group with 40 cases in each group.The control group was flushed with sodium hypochlorite stainless steel file，while the patients in the observation group were washed with Nickel-titanium instrument M3 combined with sodium hypochlorite.The biofilm growth in the two groups was compared.Results In the observation group， there were 0 cases with biofilm intact and 22 cases with complete disappearance of biofilm(55.00%)，12 cases(30.00%)were significantly better than the control group and 8 cases(20.00% ).The survival rate of bacteria in the observation group after the sodium hypochlorite flushing was 5.00% (2 cases)， which was significantly lower than that in the control group52.50%(21 cases)(P<0.05).Conclusion The nickel - titanium device M3 combined with sodium hypochlorite can better inhibit the growth of the biofilm in the root canal，exert the sterilization and disinfection functions of the sodium hypochlorite to the maximum extent，destroy the bacterial biofilm and reduce the bacterial survival rate，and can be clinically widely recommended.
, http://www.100md.com
Key words：Nickel titanium apparatus M3；Sodium hypochlorite；Endodontic biofilm
根尖周病變(periapical disease)是指发生在根管内，牙根周围组织，如牙本质、牙骨质、根尖周围的牙周膜和牙槽骨等组织之间的疾病，其主要致病因素为细菌感染[1]。研究表明，生物膜在根管内生长是慢性根尖周炎和根管再感染的主要病因，因此抑制根管内生物膜的形成尤为重要[2]。比对各种大量相关的临床资料表明，临床上常用的冲洗剂—次氯酸钠，不仅能够具有很强的杀菌效果，还能有效溶解腐败坏死组织，在一定程度上清除感染，但注射方式还有待考究[3-4]。常规不锈钢锉注射不均匀，杀菌效果还有待提高，随着医学的发展，各类医学仪器的出现，镍钛器械M3被引入到临床应用当中，目前效果反响良好。鉴于此，我院特做了此次研究，现将结果报告如下。
1资料与方法
1.1一般资料 选取我院自2015年4月～2016年4月收集的80例患有慢性根尖周病的患者作为研究对象，所有患者均经临床确诊为慢性根尖周炎，且经牙髓点活力检测为阴性。两组患者在近两年内牙齿均无外伤且无牙冠缺损严重和牙周疾病等现象，所有程序经过医院伦理委员会审核。随机将患者分为对照组和观察组两组，每组40例。其中对照组患者男23例，女17例，年龄18～63岁，平均年龄(38.34±3.21)岁；观察组患者男22例，女18例，年龄16～62岁，平均年龄(37.27±3.53)岁。两组患者一般资料对比，差异无统计学意义(P>0.05)，具有可比性。, http://www.100md.com(付爽 杨东红 宁尚波)